Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- A300-923A - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- USP15 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- The recommended shelf life for this product is 1 year from date of receipt.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human and mouse USP15 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, K-562, MCF-7, U2OS, RKO, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-USP15 antibody (Product # A300-923A; lot A300-923A-2) used for WB at 0.1 µg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of mouse and rat USP15 by western blot. Samples: Whole cell lysate (50 µg) from NIH3T3, TCMK-1, 4T1, CT26.WT, and rat C6 cells. Antibodies: Affinity purified rabbit anti-USP15 antibody A300-923A (lot A300-923A-1) used for WB at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human USP15 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-USP15 antibody A300-923A used for WB at 0.4 µg/ml (A) and 1.0 µg/ml (B) and used for IP at 3 µg/mg lysate (B). Detection: Chemiluminescence with exposure times of 3 minutes (A and B).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human and mouse USP15 by immunohistochemistry. Sample: FFPE sections of human lung carcinoma (left) and mouse renal cell carcinoma (right). Antibody: Affinity purified rabbit anti- USP15 (Cat. No. A300-923A Lot1) used at a dilution of 1:200 (1µg/ml). Detection: DAB.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human USP15 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-USP15 antibody (Product # A300-923A; lot A300-923A-2) used for IP at 6 µg per reaction. USP15 was also immunoprecipitated by a previous lot of this antibody (lot A300-923A-1). For blotting immunoprecipitated USP15, Product # A300-923A was used at 0.04 µg/mL. Detection: Chemiluminescence with an exposure time of 10 seconds.