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Western blot
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- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [2]
- Immunohistochemistry [3]
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- Product number
- NB110-40690 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB110-40690, RRID:AB_2241531
- Product name
- Rabbit Polyclonal USP15 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Ubiquitin Specific Peptidase 15 (USP15) suppresses glioblastoma cell growth via stabilization of HECTD1 E3 ligase attenuating WNT pathway activity.
OTUB1 modulates c-IAP1 stability to regulate signalling pathways.
Transgenic overexpression of USP15 in the heart induces cardiac remodeling in mice.
Oikonomaki M, Bady P, Hegi ME
Oncotarget 2017 Dec 15;8(66):110490-110502
Oncotarget 2017 Dec 15;8(66):110490-110502
OTUB1 modulates c-IAP1 stability to regulate signalling pathways.
Goncharov T, Niessen K, de Almagro MC, Izrael-Tomasevic A, Fedorova AV, Varfolomeev E, Arnott D, Deshayes K, Kirkpatrick DS, Vucic D
The EMBO journal 2013 Apr 17;32(8):1103-14
The EMBO journal 2013 Apr 17;32(8):1103-14
Transgenic overexpression of USP15 in the heart induces cardiac remodeling in mice.
Isumi Y, Hirata T, Saitoh H, Miyakawa T, Murakami K, Kudoh G, Doi H, Ishibashi K, Nakajima H
Biochemical and biophysical research communications 2011 Feb 11;405(2):216-21
Biochemical and biophysical research communications 2011 Feb 11;405(2):216-21
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: USP15 Antibody [NB110-40690] - Whole cell lysate (50 ug) from NIH3T3, TCMK-1, 4T1, CT26.WT, and rat C6 cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: USP15 Antibody [NB110-40690] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Affinity purified rabbit anti- USP15 antibody NB110-40690 used for WB at 0.4 mcg/ml (A) and 1.0 mcg/ml (B) and used for IP at 3 mcg/mg lysate (B).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: USP15 Antibody [NB110-40690] - FFPE section of human ovarian carcinoma. Affinity purified rabbit anti-USP15 used at a dilution of 1:100.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: USP15 Antibody [NB110-40690] - FFPE section of mouse teratoma. Antibody used at a dilution of 1:100. Detection: DAB staining using Immunohistochemistry Accessory Kit. Epitope Retrieval Buffer-High pH was substituted for Epitope Retrieval Buffer-Reduced pH.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: USP15 Antibody [NB110-40690] - Sections of human lung carcinoma (left) and mouse renal cell carcinoma (right). Antibody: Affinity purified rabbit anti- USP15 used at a dilution of 1:200 (1ug/ml). Detection: DAB
