Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- AF7627 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Prostaglandin E Synthase 2/ PTGES2 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human Prostaglandin E Synthase 2/PTGES2 in direct ELISAs and Western blots.
- Reactivity
- Human
- Host
- Sheep
- Conjugate
- Unconjugated
- Antigen sequence
Q9H7Z7
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Prostaglandin E Synthase 2/ PTGES2 by Western Blot. Western blot shows lysates of SW480 human colorectal adenocarcinoma cell line, COLO 205 human colorectal adenocarcinoma cell line, HepG2 human hepatocellular carcinoma cell line, A549 human lung carcinoma cell line, and human heart tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Prostaglandin E Synthase 2/PTGES2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7627) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Prostaglandin E Synthase 2/PTGES2 at approximately 30-32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Prostaglandin E Synthase 2/PTGES2 in Human Kidney. Prostaglandin E Synthase 2/PTGES2 was detected in immersion fixed paraffin-embedded sections of human kidney using Sheep Anti-Human Prostaglandin E Synthase 2/PTGES2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7627) at 0.3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Sheep IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC006). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in convoluted tubules. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.