Explore
Validate
Learn
Western blot
Immunocytochemistry
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Immunocytochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- ALX-804-816-C100 - Provider product page
- Provider
- Enzo Life Sciences
- Proper citation
- Enzo Life Sciences Cat#ALX-804-816-C100, RRID:AB_2052331
- Product name
- Promyelocytic leukemia protein (mouse) monoclonal antibody (36-1-104)
- Antibody type
- Monoclonal
- Antigen
- Recombinant protein fragment
- Reactivity
- Mouse
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 36-1-104
- Vial size
- 100 μg
- Storage
- -20°C
- Handling
- Avoid freeze/thaw cycles.
Submitted references Identification of a tumour suppressor network opposing nuclear Akt function.
PML regulates p53 stability by sequestering Mdm2 to the nucleolus.
PML is a direct p53 target that modulates p53 effector functions.
Trotman LC, Alimonti A, Scaglioni PP, Koutcher JA, Cordon-Cardo C, Pandolfi PP
Nature 2006 May 25;441(7092):523-7
Nature 2006 May 25;441(7092):523-7
PML regulates p53 stability by sequestering Mdm2 to the nucleolus.
Bernardi R, Scaglioni PP, Bergmann S, Horn HF, Vousden KH, Pandolfi PP
Nature cell biology 2004 Jul;6(7):665-72
Nature cell biology 2004 Jul;6(7):665-72
PML is a direct p53 target that modulates p53 effector functions.
de Stanchina E, Querido E, Narita M, Davuluri RV, Pandolfi PP, Ferbeyre G, Lowe SW
Molecular cell 2004 Feb 27;13(4):523-35
Molecular cell 2004 Feb 27;13(4):523-35
No comments: Submit comment
Supportive validation
- Submitted by
- Enzo Life Sciences (provider)
- Main image
- Experimental details
- Western blot analysis of endogenous PML (mouse) in different murine cell lines using MAb to PML (mouse) (36-1-104) (Prod. No. ALX-804-816). . Method: Total cell extracts (10µg) of the indicated murine cell lines were run on a SDS-gel and revealed with MAb to PML (36-1-104) (1:1'000) for 1 hour at RT followed by incubation with HRP-coupled goat-anti mouse antibody (1:4'000) (Jackson Laboratories). The arrows indicate endogenous PML. Note: the band PML* probably represents an isoform of PML.
