12525-1-AP

antibody from Invitrogen Antibodies

Targeting: IRF2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

12525-1-AP

Provider

Invitrogen Antibodies

Product name

IRF2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MPVERMRMR PWLEEQINSN TIPGLKWLNK EKKIFQIPWM HAARHGWDVE KDAPLFRNWA IHTGKHQPGV DKPDPKTWKA NFRCAMNSLP DIEEVKDKSI KKGNNAFRVY RMLPLSERPS KKGKKPKTEK EDKVKHIKQE PVESSLGLSN GVSDLSPEYA VLTSTIKNEV DSTVNIIVVG QSHLDSNIEN QEIVTNPPDI CQVVEVTTES DEQPVSMSEL YPLQISPVSS YAESETTDSV PSDEESAEGR PHWRKRNIEG KQYLSNMGTR GSYLLPGMAS FVTSNKPDLQ VTIKEESNPV PYNSSWPPFQ DLPLSSSMTP ASSSSRPDRE TRASVIKKTS DITQARVKSC (1-349 aa encoded by BC015803)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.22 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

COLO 320 cells were subjected to SDS PAGE followed by western blot with 12525-1-AP (IRF2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse colon tissue were subjected to SDS PAGE followed by western blot with 12525-1-AP (IRF2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Jurkat cells were subjected to SDS PAGE followed by western blot with 12525-1-AP (IRF2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using IRF2 antibody (Product # 12525-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human colon cancer using 12525-1-AP (IRF2 antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human colon cancer using 12525-1-AP (IRF2 antibody) at dilution of 1:50 (under 40x lens).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-IRF2 (IP:12525-1-AP, 4ug; Detection:12525-1-AP 1:500) with Jurkat cells lysate 2800ug.