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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [3]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-41715 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RPL13 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Peptide sequence: KKEKARVITE EEKNFKAFAS LRMARANARL FGIRAKRAKE AAEQDVEKKK
- Concentration
- 0.5 mg/mL
Submitted references Age-dependent shift in the de novo proteome accompanies pathogenesis in an Alzheimer's disease mouse model.
Elder MK, Erdjument-Bromage H, Oliveira MM, Mamcarz M, Neubert TA, Klann E
Communications biology 2021 Jun 30;4(1):823
Communications biology 2021 Jun 30;4(1):823
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Western blot analysis of human Jurkat cell lysate using an anti-RPL13 polyclonal antibody (Product # PA5-41715).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of human HEK293T cells using an anti-RPL13 polyclonal antibody (Product # PA5-41715).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of human MCF7 cells using an anti-RPL13 polyclonal antibody (Product # PA5-41715).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (paraffin-embedded) analysis of human lung tissue using an anti-RPL13 polyclonal antibody (Product # PA5-41715).
Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Fig. 4 Candidate proteins identified with BONLAC screen of de novo proteome exhibit altered expression levels in APP/PS1 mice. a Western blot quantitation of candidate proteins selected from BONLAC screen in 3-5-month-old wild-type (WT; light bars) and APP/PS1 (dark bars) mice, normalized in- lane to total protein (as assessed by MemCode or Ponceau stain [note only a portion of the entire analyzed lane is presented in the figure]) and expressed relative to age-matched WT (Number of biologically independent animals samples: APP 3-5 month n = 8, 12+ month n = 6; EAAT1 n = 3; Gap-43 n = 6; Hsp1a1 n = 3; Rpl13 3-5 month n = 9, 12+ month n = 12; Rpl18: n = 7); representative western blots show selected protein levels in WT and APP/PS1 mouse hippocampal lysates. b Representative western blot and quantitation of candidate proteins selected from BONLAC screen in 12+-month-old WT and APP/PS1 mice. c Comparison table showing average fold change in de novo synthesis of candidate proteins in young and aged APP/PS1 vs WT littermates as identified by BONLAC (young n = 5 biologically independent samples; aged n = 7 biologically independent samples) compared to change in total expression quantified by western blot. Statistical significance calculated between APP/PS1 and WT samples using unpaired two-tailed t -tests; young APP: t = 3.378, 95% confidence interval = 0.4571-2.047, effect size = 1.252 +- 0.3707, df = 14, p = 0.0045; young EAAT1: t = 2.945, 95% confidence interval = 0.0146-0.4966,
