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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [5]
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- Validations
- Flow cytometry [1]
- Other assay [1]
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- Product number
- 12-0666-42 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD66b Monoclonal Antibody (G10F5), PE, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: This G10F5 monoclonal antibody reacts with human CD66b, one of four members of the CD66 family of granulocyte-specific activation antigens. Expressed on neutrophils and eosinophils, CD66b is upregulated following stimulation. This glycosyl-phosphatidylinositol-linked cell surface receptor plays a role in regulating neutrophil activation and adhesion to fibronectin, galectin-3, and E-selectin.
- Conjugate
- Yellow dye
- Antibody clone number
- G10F5
- Concentration
- 5 µL/Test
Submitted references Distinct clinical and immunological profiles of patients with evidence of SARS-CoV-2 infection in sub-Saharan Africa.
Loss of testosterone impairs anti-tumor neutrophil function.
Lipopolysaccharides from Microcystis Cyanobacteria-Dominated Water Bloom and from Laboratory Cultures Trigger Human Immune Innate Response.
Tumor cell-released autophagosomes (TRAP) enhance apoptosis and immunosuppressive functions of neutrophils.
Neonatal myeloid derived suppressor cells show reduced apoptosis and immunosuppressive activity upon infection with Escherichia coli.
Morton B, Barnes KG, Anscombe C, Jere K, Matambo P, Mandolo J, Kamng'ona R, Brown C, Nyirenda J, Phiri T, Banda NP, Van Der Veer C, Mndolo KS, Mponda K, Rylance J, Phiri C, Mallewa J, Nyirenda M, Katha G, Kambiya P, Jafali J, Mwandumba HC, Gordon SB, Blantyre COVID-19 Consortium, Cornick J, Jambo KC
Nature communications 2021 Jun 11;12(1):3554
Nature communications 2021 Jun 11;12(1):3554
Loss of testosterone impairs anti-tumor neutrophil function.
Markman JL, Porritt RA, Wakita D, Lane ME, Martinon D, Noval Rivas M, Luu M, Posadas EM, Crother TR, Arditi M
Nature communications 2020 Mar 31;11(1):1613
Nature communications 2020 Mar 31;11(1):1613
Lipopolysaccharides from Microcystis Cyanobacteria-Dominated Water Bloom and from Laboratory Cultures Trigger Human Immune Innate Response.
Moosová Z, Šindlerová L, Ambrůzová B, Ambrožová G, Vašíček O, Velki M, Babica P, Kubala L
Toxins 2019 Apr 11;11(4)
Toxins 2019 Apr 11;11(4)
Tumor cell-released autophagosomes (TRAP) enhance apoptosis and immunosuppressive functions of neutrophils.
Gao R, Ma J, Wen Z, Yang P, Zhao J, Xue M, Chen Y, Aldarouish M, Hu HM, Zhu XJ, Pan N, Wang LX
Oncoimmunology 2018;7(6):e1438108
Oncoimmunology 2018;7(6):e1438108
Neonatal myeloid derived suppressor cells show reduced apoptosis and immunosuppressive activity upon infection with Escherichia coli.
Leiber A, Schwarz J, Köstlin N, Spring B, Fehrenbach B, Katava N, Poets CF, Gille C
European journal of immunology 2017 Jun;47(6):1009-1021
European journal of immunology 2017 Jun;47(6):1009-1021
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of normal human peripheral blood cells with Mouse IgM Isotype Control PE (Product # 12-4752) (blue histogram) or Anti-Human CD66b PE (purple histogram). Cells in the granulocyte gate were used for analysis.
- Conjugate
- Yellow dye
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 5 Analysis of neutrophils from control or androgen deprivation therapy (ADT) treated patients. a Percent of circulating neutrophils from control or ADT treated prostate cancer patients that have a mature, segmented appearance morphologically. b Percent Cd11b + CD66b + CD16 lo neutrophils. c Percent myeloid derived suppressor cell (MDSC)-like neutrophils defined as HLA-DR - CD14 - CD33 + CD15 + . d Percent fold change in migration (percent crystal violet positive area of transwell stimulated with a chemoattractant divided by percent area without chemoattractant). e Percent of unstimulated neutrophils producing DHR123. f Percent of neutrophils producing DHR123 following PMA stimulation. g Percent myeloperoxidase + (MPO + ) neutrophils. h Percent of plated netosising neutrophils at 1 and 3 h following purification. Data in a - h are mean +- s.e.m., * p < 0.05, ** p < 0.01, *** p < 0.001 by multivariable linear regression using ADT as its main predictor and adjusting for presence of prostatectomy, age, race, and current status; control prostate cancer patients ( n = 8 in a , c , g and n = 7 in b , d , e , f , h ) and prostate cancer pati e nts receiving ADT ( n = 22 in a , c , g and n = 21 in b , d , e , f , h ).
- Conjugate
- Yellow dye
