DM1229

antibody from Acris Antibodies GmbH

Targeting: CEACAM8 CD66b, CGM6

ELISA (Recommended by provider)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

DM1229

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#DM1229, RRID:AB_10651229

Product name

anti CD66b / CEACAM8

Antibody type

Monoclonal

Antigen

Genetic immunisation with cDNA encoding Human CEACAM8.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

GM2H6

Vial size

0.1 mg

Concentration

2.0 mg/ml

References

Submitted references

CD66b regulates adhesion and activation of human eosinophils.

Yoon J, Terada A, Kita H
Journal of immunology (Baltimore, Md. : 1950) 2007 Dec 15;179(12):8454-62

Flow cytometry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Figure.1: FACS analysis of BOSC23 cells using GM2H6 antibody (Cat.#DM1229). BOSC23 cells were transiently transfected with an expression vector encoding either CEACAM8 (Red curve) or an irrelevant protein (control transfectant). Binding of GM-0512 was detected with a PE conjugated secondary antibody. A positive signal was obtained only with CEACAM8 transfected cells.

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Figure 2: BOSC23 cells were transiently transfected with expression vectors containing either the cDNA of CEACAM1, CEACAM3-8 or PSG. The latter expressed as a membrane bound fusion protein. Expression of the constructswas tested with monoclonal antibodies known to recognize the corresponding proteins (CEACAM1,3,4,5 and 6: D14HD11; CEACAM7: BAC2; CEACAM8:Tet2; PSG: BAP3; Green curves). An irrelevant monoclonal antibody served as a negative control (black curves). For specificity testing, protein G-purified GM-2H6 was tested on all CEACAM transfectants. A positive signal wasobtained only with CEACAM8 transfected cells (Red curve).