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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
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Validation data
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- Product number
- R31082 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- 53BP1 Antibody
- Antibody type
- Polyclonal
- Antigen
- An amino acid sequence from the C-terminus of human 53BP1 (ERIGFKQHPKYKHDYVSH) was used as the immunogen for this 53BP1 antibody (100% mouse homology).
- Description
- Antigen affinity purified antibody
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 µg
- Concentration
- Lyophilized; resuspend with 200 ul for 0.5 mg/ml
- Storage
- After reconstitution, the 53BP1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 53BP1 antibody and Lane 1: human 293T; 2: (h) U20S; 3: mouse HEPA cell lysate. Predicted/observed molecular weight: ~214kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- ICC testing of 53BP1 antibody and A549 cells
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC-P: 53BP1 antibody testing of human intestinal cancer tissue. HIER: steamed with pH6 citrate buffer.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC-P: 53BP1 antibody testing of rat brain tissue. HIER: steamed with pH6 citrate buffer.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC-P: 53BP1 antibody testing of mouse brain tissue. HIER: steamed with pH6 citrate buffer.
