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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Immunocytochemistry [1]
- Immunoprecipitation [1]
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- Product number
- TA328075 - Provider product page
- Provider
- OriGene
- Product name
- Mouse Monoclonal anti-Cytochrome c Antibody
- Antibody type
- Monoclonal
- Description
- Mouse Monoclonal anti-Cytochrome c Antibody
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- COX15
- Isotype
- IgG
- Antibody clone number
- 6H2.B4
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HeLa cells were fixed with 1% paraformaldehyde (PFA) for 10 minutes, permeabilized with 0.5% Triton X-100 for 10 minutes, and blocked with 5% FBS for 30 minutes. Then the cells were intracellularly stained with 5 ug/ml of purified cytochrome c (clone 6H2.B4) in blocking buffer overnight at 4°C, and followed by DyLight™ 594 anti-mouse IgG (red) staining for 2 hours at 4°C. Nuclei were counterstained with DAPI and are shown in blue. The image was captured with 40X objective.
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Cytochrome c was immunoprecipitated from Hela cell extract (1% NP-40) using 2-4 ug 6H2.B4 antibody/1 x107 cell equivalents. Immunoprecipitates were resolved by electrophoresis, transferred to nitrocellulose, and probed with the 7H8.2C12 anti-cytochrome c antibody. In addition to the specific 15 kD cytochrome c band immunoprecipitated by 6H2.B4, heavy and light immunoglobulin chains are recognized by the goat anti-mouse secondary antibody.
- Validation comment
- IP
