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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- TA324502 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit polyclonal SP140 Antibody (Center)
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal SP140 Antibody (Center)
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- SP140
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 400 µl
- Concentration
- 0.5 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- SP140 Antibody (Center) (Cat. #TA324502) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the SP140 antibody detected the SP140 protein (arrow).
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- IF image of Hela cell stained with SP140 Antibody (Center)(Cat#TA324502).Hela cells were incubated with SP140 primary antibody (1:25, 1 h at 37?). For secondary antibody, Alexa Fluor? 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor? 555 (red) conjugated Phalloidin (7 units/ml). Nuclei were counterstained with DAPI (blue) . SP140 immunoreactivity is localized to Cytoplasm and Nucleus significantly.
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- SP140 Antibody (Center) (Cat. #TA324502)immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SP140 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
- Validation comment
- IHC
