NBP2-12763

antibody from Novus Biologicals

Targeting: GMEB2 KIAA1269, P79PIF, PIF79

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP2-12763

Provider

Novus Biologicals

Product name

Rabbit Polyclonal GMEB2 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 ul

Concentration

1.0 mg/ml

Storage

Store at 4C. Do not freeze.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: GMEB2 Antibody [NBP2-12763] - Detection of human GMEB2 by western blot and immunoprecipitation. Samples: Whole cell lysate from Jurkat (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug) and HeLa (H; 50 ug) cells. Antibodies: Affinity purified rabbit anti-GMEB2 antibody NBP2-12763 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. GMEB2 was also immunoprecipitated by rabbit anti-GMEB2 antibody NBP2-12762, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).

Immunoprecipitation

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunoprecipitation: GMEB2 Antibody [NBP2-12763] - Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from Jurkat cells. Antibodies: Affinity purified rabbit anti-GMEB2 antibody NBP2-12762 used for IP at 6 ug/mg lysate. GMEB2 was also immunoprecipitated by rabbit anti-GMEB2 antibody NBP2-12763, which recognizes a downstream epitope. For blotting immunoprecipitated GMEB2, NBP2-12763 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.