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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Immunohistochemistry [1]
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- Product number
- AF194 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal Frizzled-4 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects mouse Frizzled-4 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant mouse (rm) Frizzled-8 and less than 2% cross-reactivity with rmFrizzled-3 and rmFrizzled-7 is observed.
- Reactivity
- Mouse
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references MicroRNAs regulate ocular neovascularization.
Amyloid-beta binds to the extracellular cysteine-rich domain of Frizzled and inhibits Wnt/beta-catenin signaling.
Wnt5a signaling induces proliferation and survival of endothelial cells in vitro and expression of MMP-1 and Tie-2.
Shen J, Yang X, Xie B, Chen Y, Swaim M, Hackett SF, Campochiaro PA
Molecular therapy : the journal of the American Society of Gene Therapy 2008 Jul;16(7):1208-16
Molecular therapy : the journal of the American Society of Gene Therapy 2008 Jul;16(7):1208-16
Amyloid-beta binds to the extracellular cysteine-rich domain of Frizzled and inhibits Wnt/beta-catenin signaling.
Magdesian MH, Carvalho MM, Mendes FA, Saraiva LM, Juliano MA, Juliano L, Garcia-Abreu J, Ferreira ST
The Journal of biological chemistry 2008 Apr 4;283(14):9359-68
The Journal of biological chemistry 2008 Apr 4;283(14):9359-68
Wnt5a signaling induces proliferation and survival of endothelial cells in vitro and expression of MMP-1 and Tie-2.
Masckauchán TN, Agalliu D, Vorontchikhina M, Ahn A, Parmalee NL, Li CM, Khoo A, Tycko B, Brown AM, Kitajewski J
Molecular biology of the cell 2006 Dec;17(12):5163-72
Molecular biology of the cell 2006 Dec;17(12):5163-72
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Frizzled-4 in Embryonic Mouse Neuronal Tissue. Frizzled-4 was detected in immersion fixed frozen sections of embryonic mouse neuronal tissue (E11) using 15 µg/mL Goat Anti-Mouse Frizzled-4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF194) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the plasma membrane and cytoplasm of neuronal cells in the midbrain. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
