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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
- Other assay [1]
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- Product number
- PA5-27109 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TIP30 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Recommended positive controls: A431, HeLa.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using TIP30 Polyclonal Antibody (Product # PA5-27109). Sample (30 µg of whole cell lysate). Lane A: A431. Lane B: HeLa. 12% SDS PAGE. TIP30 Polyclonal Antibody (Product # PA5-27109) diluted at 1:5,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of TIP30 in paraformaldehyde-fixed HeLa cells using a TIP30 polyclonal antibody (Product # PA5-27109) (Green) at a 1:500 dilution. Alpha-tubulin filaments were labeled with Product # PA5-29281 (Red) at a 1:2000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human breast cancer, using TIP30 (Product # PA5-27109) antibody at 1:250 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- TIP30 Polyclonal Antibody detects TIP30 protein at cytoplasm in mouse muscle by immunohistochemical analysis. Sample: Paraffin-embedded mouse muscle. TIP30 Polyclonal Antibody (Product # PA5-27109) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- TIP30 Polyclonal Antibody detects TIP30 protein on human gastric cancer by immunohistochemical analysis. Sample: Paraffin-embedded gastric cancer. TIP30 Polyclonal Antibody (Product # PA5-27109) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- TIP30 Polyclonal Antibody immunoprecipitates TIP30 protein in IP experiments. IP Sample: hela whole cell lysate/extract A. 40 µg hela whole cell lysate/extract B. Control with 2 µg of preimmune rabbit IgG C. Immunoprecipitation of TIP30 protein by 2 µg of TIP30 Polyclonal Antibody (Product # PA5-27109) 10% SDS-PAGE The immunoprecipitated TIP30 protein was detected by TIP30 Polyclonal Antibody (Product # PA5-27109) diluted at 1:1,000. Anti-rabbit IgG was used as a secondary reagent.
