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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- AM08448PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AM08448PU-N, RRID:AB_2035132
- Product name
- anti APAF1
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide corresponding to a region near the carboxy terminus of Human Apaf1 protein
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 2E10
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/ml (by UV absorbance at 280 nm)
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot using anti-Apaf1 antibody shows detection of a single band at ~130 kDa corresponding to Apaf1 in K562 cell lysate. The primary antibody was used at 250 ng/ml (lane A) and 500 ng/ml (lane B).
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunohistochemical staining using monoclonal anti-Apaf1 antibody at 0.5 µg/ml shows detection of Apaf1 in K562 cells. Cells were fixed in 4% paraformaldehyde.
