M00889

antibody from Boster Biological Technology

Targeting: APAF1 APAF-1, CED4

Western blot (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

M00889

Provider

Boster Biological Technology

Product name

Anti-APAF1 Rabbit Monoclonal Antibody

Antibody type

Monoclonal

Description

Monoclonal antibody for APAF-1/APAF1 detection. Host: Rabbit.Size: 100ug/vial. Tested applications: IF, IHC, ICC, WB. Reactive species: Human, Mouse APAF-1/APAF1 information: Molecular Weight: 141840 MW; Subcellular Localization: Cytoplasm ; Tissue Specificity: Ubiquitous. Highest levels of expression in adult spleen and peripheral blood leukocytes, and in fetal brain, kidney and lung. Isoform 1 is expressed in heart, kidney and liver.

Reactivity

Human, Mouse

Host

Rabbit

Antibody clone number

BHF-1

Vial size

100ug/vial

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Storage

At -20°C for one year. Avoid repeated freezing and thawing.

Immunohistochemistry

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded mouse kidney, using APAF1 Antibody (M00889)APAF1 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-APAF1 Antibody (M00889)overnight at 4?? Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37?? The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Additional image