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Western blot
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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [5]
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- Validations
- Immunocytochemistry [2]
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- Product number
- ALX-804-348-C100 - Provider product page
- Provider
- Enzo Life Sciences
- Proper citation
- Enzo Life Sciences Cat#ALX-804-348-C100, RRID:AB_2050726
- Product name
- Apaf-1 (human) monoclonal antibody (2E12)
- Antibody type
- Monoclonal
- Antigen
- Recombinant protein fragment
- Description
- Protein G-affinity purified.
- Reactivity
- Human
- Host
- Rat
- Isotype
- IgG
- Antibody clone number
- 2E12
- Vial size
- 100 μg
- Storage
- -20°C
- Handling
- Avoid freeze/thaw cycles.
Submitted references Induced inhibition of ischemic/hypoxic injury by APIP, a novel Apaf-1-interacting protein.
BOK and NOXA are essential mediators of p53-dependent apoptosis.
Anti-Fas antibody-induced apoptosis and its signal transduction in human gastric carcinoma cell lines.
Pro-apoptotic apoptosis protease-activating factor 1 (Apaf-1) has a cytoplasmic localization distinct from Bcl-2 or Bcl-x(L).
Bcl-2 family members do not inhibit apoptosis by binding the caspase activator Apaf-1.
Cho DH, Hong YM, Lee HJ, Woo HN, Pyo JO, Mak TW, Jung YK
The Journal of biological chemistry 2004 Sep 17;279(38):39942-50
The Journal of biological chemistry 2004 Sep 17;279(38):39942-50
BOK and NOXA are essential mediators of p53-dependent apoptosis.
Yakovlev AG, Di Giovanni S, Wang G, Liu W, Stoica B, Faden AI
The Journal of biological chemistry 2004 Jul 2;279(27):28367-74
The Journal of biological chemistry 2004 Jul 2;279(27):28367-74
Anti-Fas antibody-induced apoptosis and its signal transduction in human gastric carcinoma cell lines.
Adachi K, Osaki M, Kase S, Takeda A, Ito H
International journal of oncology 2003 Sep;23(3):713-9
International journal of oncology 2003 Sep;23(3):713-9
Pro-apoptotic apoptosis protease-activating factor 1 (Apaf-1) has a cytoplasmic localization distinct from Bcl-2 or Bcl-x(L).
Hausmann G, O'Reilly LA, van Driel R, Beaumont JG, Strasser A, Adams JM, Huang DC
The Journal of cell biology 2000 May 1;149(3):623-34
The Journal of cell biology 2000 May 1;149(3):623-34
Bcl-2 family members do not inhibit apoptosis by binding the caspase activator Apaf-1.
Moriishi K, Huang DC, Cory S, Adams JM
Proceedings of the National Academy of Sciences of the United States of America 1999 Aug 17;96(17):9683-8
Proceedings of the National Academy of Sciences of the United States of America 1999 Aug 17;96(17):9683-8
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Supportive validation
- Submitted by
- Enzo Life Sciences (provider)
- Main image
- Experimental details
- Western blot analysis with anti-Apaf-1 (human) MAb (clone 2E12) revealing endogenous Apaf-1. Lysates from 10E6 HeLa, MCF-7, 293T, COS, Jurkat, and activated primary human T cells were probed with clone 2E12. COS (monkey) cells were used as a negative control.
- Submitted by
- Enzo Life Sciences (provider)
- Main image
- Experimental details
- Figures: Species cross-reactivity of MAb to Apaf-1 (clone 2E12) (ALX-804-348) by Western blot. A: Lysates from 1-day-old mouse brain (lane 1), adult mouse brain (lane 2) and SH-SY5Y human neuroblastoma cell line (lane 3) were probed with clone 2E12. B: Lysates from 2-days-old rat brain (lane 1) and adult rat brain (lane 2) were probed with clone 2E12. . . Results: 2E12 is human specific and does not cross-react with mouse or rat Apaf-1.
