MA5-34873

antibody from Invitrogen Antibodies

Targeting: BCAM CD239, LU

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-34873

Provider

Invitrogen Antibodies

Product name

BCAM Recombinant Rabbit Monoclonal Antibody (JE51-07)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Description

Positive Control: A431 cell, human kidney tissue, human placenta tissue, JAR.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Antibody clone number

JE51-07

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles, store in dark

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of CD239 in A431 cell lysate. Samples were transferred to PVDF membrane, blocked with 5% BSA (1 hour), incubated with CD239 monoclonal antibody (Product # MA5-34873), at a dilution of 1:500, followed by Goat Anti-Rabbit IgG-HRP (1 hour) with a dilution of 1:5000.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of CD239 in paraffin-embedded human placenta tissue. Samples were heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4, 20 minutes) and blocked in 5% BSA (30 min, room temperature), incubated with CD239 monoclonal antibody (Product # MA5-34873) at a dilution of 1:200 (30 min, room temperature), and followed by HRP conjugate, DAB and hematoxylin (mounted with DPX).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of CD239 in paraffin-embedded human kidney tissue. Samples were heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4, 20 minutes) and blocked in 5% BSA (30 min, room temperature), incubated with CD239 monoclonal antibody (Product # MA5-34873) at a dilution of 1:200 (30 min, room temperature), and followed by HRP conjugate, DAB and hematoxylin (mounted with DPX).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of CD239 in JAR cells, unlabelled sample (control; cells without incubation with primary antibody; black). Samples were incubated with CD239 monoclonal antibody (Product # MA5-34873) at a dilution of 1:50, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG with a dilution of 1:1000 (30 min).