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- Antibody Data
- Antigen structure
- References [4]
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- Validations
- Flow cytometry [1]
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- Product number
- MAB1481 - Provider product page
- Provider
- R&D Systems
- Product name
- Human BCAM Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human BCAM in ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) ALCAM, rhEpCAM, recombinant mouse (rm) MAdCAM-1, rhMCAM, rhNCAM-L1, rmOCAM, or rmTROP-2 is observed.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
CAA58449- Isotype
- IgG
- Antibody clone number
- 87207
- Vial size
- 500 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references An Anti-Human Lutheran Glycoprotein Phage Antibody Inhibits Cell Migration on Laminin-511: Epitope Mapping of the Antibody.
Hydroxycarbamide decreases sickle reticulocyte adhesion to resting endothelium by inhibiting endothelial lutheran/basal cell adhesion molecule (Lu/BCAM) through phosphodiesterase 4A activation.
The lutheran/basal cell adhesion molecule promotes tumor cell migration by modulating integrin-mediated cell attachment to laminin-511 protein.
An antibody to the lutheran glycoprotein (Lu) recognizing the LU4 blood type variant inhibits cell adhesion to laminin α5.
Enomoto-Okawa Y, Maeda Y, Harashima N, Sugawara Y, Katagiri F, Hozumi K, Hui KM, Nomizu M, Ito Y, Kikkawa Y
PloS one 2017;12(1):e0167860
PloS one 2017;12(1):e0167860
Hydroxycarbamide decreases sickle reticulocyte adhesion to resting endothelium by inhibiting endothelial lutheran/basal cell adhesion molecule (Lu/BCAM) through phosphodiesterase 4A activation.
Chaar V, Laurance S, Lapoumeroulie C, Cochet S, De Grandis M, Colin Y, Elion J, Le Van Kim C, El Nemer W
The Journal of biological chemistry 2014 Apr 18;289(16):11512-11521
The Journal of biological chemistry 2014 Apr 18;289(16):11512-11521
The lutheran/basal cell adhesion molecule promotes tumor cell migration by modulating integrin-mediated cell attachment to laminin-511 protein.
Kikkawa Y, Ogawa T, Sudo R, Yamada Y, Katagiri F, Hozumi K, Nomizu M, Miner JH
The Journal of biological chemistry 2013 Oct 25;288(43):30990-1001
The Journal of biological chemistry 2013 Oct 25;288(43):30990-1001
An antibody to the lutheran glycoprotein (Lu) recognizing the LU4 blood type variant inhibits cell adhesion to laminin α5.
Kikkawa Y, Miwa T, Tohara Y, Hamakubo T, Nomizu M
PloS one 2011;6(8):e23329
PloS one 2011;6(8):e23329
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of BCAM in Huh-7 Human Cell Line by Flow Cytometry. Huh-7 human hepatoma cell line was stained with Mouse Anti-Human BCAM Monoclonal Antibody (Catalog # MAB1481, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).
