14960-1-AP

antibody from Invitrogen Antibodies

Targeting: PABPC4 APP-1, iPABP

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

14960-1-AP

Provider

Invitrogen Antibodies

Product name

PABPC4 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: QERISRYQG VNLYIKNLDD TIDDEKLRKE FSPFGSITSA KVMLEDGRSK GFGFVCFSSP EEATKAVTEM NGRIVGSKPL YVALAQRKEE RKAHLTNQYM QRVAGMRALP ANAILNQFQP AAGGYFVPAV PQAQGRPPYY TPNQLAQMRP NPRWQQGGRP QGFQGMPSAI RQSGPRPTLR HLAPTGNAPA SRGLPTTTQR VGVPTAVQNL APRAAVAAAA PRAVAPYKYA SSVRSPHPAI QPLQAPQPAV HVQGQEPLTA SMLAAAPPQE QKQMLGERLF PLIQTMHSNL AGKITGMLLE IDNSELLHML ESPESLRSKV DEAVAVLQAH HAKKEAAQKV GAVAAATS (285-631 aa encoded by BC065540)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.33 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Jurkat cells were subjected to SDS PAGE followed by western blot with 14960-1-AP (PABPC4 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse heart tissue were subjected to SDS PAGE followed by western blot with 14960-1-AP (PABPC4 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Hela cells, using PABPC4 antibody 14960-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IGG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Ethacrynic acid treated HepG2 cells using 14960-1-AP (PABPC4 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human gliomas using 14960-1-AP (PABPC4 antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human gliomas using 14960-1-AP (PABPC4 antibody) at dilution of 1:50 (under 40x lens).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-PABPC4 (IP:14960-1-AP, 4ug; Detection:14960-1-AP 1:500) with mouse heart tissue lysate 4000ug.