H00010274-M01

antibody from Abnova Corporation

Targeting: STAG1 SA-1, SA1, SCC3A

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00010274-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00010274-M01, RRID:AB_509194

Product name

STAG1 monoclonal antibody (M01), clone 2E9

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a partial recombinant STAG1.

Antigen sequence

LPAPQLTSTVLRENSRPMGDQIQEPESEHGSEPDF
LHNRGLMEEDAEPIFEDVMMSSRSQLEDMNEEFED
TMVIDLPPSRNRRERAELRPDFFDSAAIIEDDSGF
GMPMF


Isotype

IgG

Antibody clone number

2E9

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

STAG1 monoclonal antibody (M01), clone 2E9 Western Blot analysis of STAG1 expression in HeLa ( Cat # L013V1 ).

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged STAG1 is approximately 0.03ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence of monoclonal antibody to STAG1 on HeLa cell. [antibody concentration 10 ug/ml]

Validation comment

Immunofluorescence

Protocol

Protocol

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunoperoxidase of monoclonal antibody to STAG1 on formalin-fixed paraffin-embedded human smooth muscle. [antibody concentration 3 ug/ml]

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Protocol

Protocol