Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- A300-156A - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SA1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- The recommended shelf life for this product is 1 year from date of receipt.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human and mouse SA1 by western blot. Samples: Whole cell lysate (15 µg) from HeLa, MCF-7, HEK293T, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SA1 antibody (Product # A300-156A; lot A300-156A-2) used for WB at 0.1 µg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human SA1 by western blot. Sample: Nuclear extract from ~500,000 HeLa cells. Antibody: Affinity purified goat anti-SA1 (Cat. No. A300-156A) used at 0.5 µg/ml. Detection: Chemiluminescence with 15 sec. exposure.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Localization of human SA1. Sample: HeLa cells that were extracted for 5 min at 4C in 0.5% Triton in CSK buffer. Antibody: Affinity purified goat anti-SA1 (BL140G; A300-156A) used at 1 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human SA1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SA1 antibody (Product # A300-156A; lot A300-156A-2) used for IP at 6 µg per reaction. SA1 was also immunoprecipitated by rabbit anti-SA1 antibody (Product # A302-579A). For blotting immunoprecipitated SA1 (Product # A300-156A) was used at 0.1 µg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.