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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunohistochemistry [1]
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- Product number
- MAB12252 - Provider product page
- Provider
- Abnova Corporation
- Product name
- HOXB4 monoclonal antibody, clone 3A2F2
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against partial recombinant human HOXB4.
- Isotype
- IgG
- Antibody clone number
- 3A2F2
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of human HOXB4 (AA: 16-251) recombinant protein (Expected MW is 51.9 kDa) with HOXB4 monoclonal antibody, clone 3A2F2 (Cat# MAB12252) at 1:500-1:2000 dilution.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of Lane 1: Negative control [HEK293 cell lysate]; Lane 2: Over-expression lysate [HOXB4 (AA: 16-251)-hIgGFc transfected HEK293 cells] with HOXB4 monoclonal antibody, clone 3A2F2 (Cat# MAB12252) at 1:500-1:2000 dilution.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- ELISA analysis of HOXB4 monoclonal antibody, clone 3A2F2 (Cat# MAB12252) at 1:10000 dilution.
- Validation comment
- ELISA
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunohistochemical staining of human rectum cancer tissues (A) and colon cancer tissues (B) with HOXB4 monoclonal antibody, clone 3A2F2 (Cat# MAB12252) at 1:200-1:1000 dilution.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
