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Western blot
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Flow cytometry [1]
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- Product number
- AF4388 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Sheep Polyclonal IL-20 R beta/FNDC6 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects mouse IL-20 R beta in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 30% cross-reactivity with recombinant human IL-20 R beta is observed.
- Reactivity
- Mouse
- Host
- Sheep
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Interleukin-20 targets podocytes and is upregulated in experimental murine diabetic nephropathy.
Hsu YH, Li HH, Sung JM, Chen WY, Hou YC, Weng YH, Lai WT, Wu CH, Chang MS
Experimental & molecular medicine 2017 Mar 31;49(3):e310
Experimental & molecular medicine 2017 Mar 31;49(3):e310
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Detection of IL-20 R beta in bEnd.3 Mouse Cell Line by Flow Cytometry. bEnd.3 mouse endothelioma cell line was stained with Mouse IL-20 R beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4388, filled histogram) or control antibody (Catalog # 5-001-A, open histogram), followed by NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL010).
