Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [7]
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Validation data
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- Product number
- NBP1-81321 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-81321, RRID:AB_11019730
- Product name
- Rabbit Polyclonal GRHL1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human GRHL1 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references ARID1A determines luminal identity and therapeutic response in estrogen-receptor-positive breast cancer.
Suppressor gene GRHL1 is associated with prognosis in patients with oesophageal squamous cell carcinoma.
EGFR regulation of epidermal barrier function.
Xu G, Chhangawala S, Cocco E, Razavi P, Cai Y, Otto JE, Ferrando L, Selenica P, Ladewig E, Chan C, Da Cruz Paula A, Witkin M, Cheng Y, Park J, Serna-Tamayo C, Zhao H, Wu F, Sallaku M, Qu X, Zhao A, Collings CK, D'Avino AR, Jhaveri K, Koche R, Levine RL, Reis-Filho JS, Kadoch C, Scaltriti M, Leslie CS, Baselga J, Toska E
Nature genetics 2020 Feb;52(2):198-207
Nature genetics 2020 Feb;52(2):198-207
Suppressor gene GRHL1 is associated with prognosis in patients with oesophageal squamous cell carcinoma.
Li M, Li Z, Guan X, Qin Y
Oncology letters 2019 May;17(5):4313-4320
Oncology letters 2019 May;17(5):4313-4320
EGFR regulation of epidermal barrier function.
Tran QT, Kennedy LH, Leon Carrion S, Bodreddigari S, Goodwin SB, Sutter CH, Sutter TR
Physiological genomics 2012 Apr 15;44(8):455-69
Physiological genomics 2012 Apr 15;44(8):455-69
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: GRHL1 Antibody [NBP1-81321] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: GRHL1 Antibody [NBP1-81321] - Analysis in human cell line BEWO.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: GRHL1 Antibody [NBP1-81321] - Staining of human esophagus shows strong nuclear positivity in squamous epithelial cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: GRHL1 Antibody [NBP1-81321] - Staining of human esophagus shows moderate to strong nuclear positivity in squamous epithelial cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: GRHL1 Antibody [NBP1-81321] - Staining of human esophagus, placenta, skeletal muscle and skin using Anti-GRHL1 antibody NBP1-81321 (A) shows similar protein distribution across tissues to independent antibody NBP1-81322 (B).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: GRHL1 Antibody [NBP1-81321] - Staining of human placenta shows strong nuclear positivity in trophoblastic cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: GRHL1 Antibody [NBP1-81321] - Staining of human skeletal muscle shows no positivity in myocytes as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: GRHL1 Antibody [NBP1-81321] - Staining of human skin shows moderate to strong nuclear positivity in squamous epithelial cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: GRHL1 Antibody [NBP1-81321] - Staining in human skin and skeletal muscle tissues using NBP1-81321 antibody. Corresponding GRHL1 RNA-seq data are presented for the same tissues.