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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- TA319375 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit polyclonal anti-NRF1 antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal anti-NRF1 antibody
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- Nrf1
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 500 µg
- Concentration
- 2.3 mg/mL
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- WB using anti-NRF1 antibody shows detection of a 67-kDa band corresponding to human NRF1 in a (lane 1) HeLa nuclear extract and (lane 2) whole cell lysate (molecular weight marker not shown). Approx. 10 ug of each lysate was separated by SDS-PAGE and transferred onto nitrocellulose. The blot was incubated with a 1:500 dilution of the antibody at RT for 1 h followed by detection using IRDye?700 labeled Goat-a-Rabbit IgG [H&L] (611-130-122) diluted 1:2,500.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Affinity Purified anti-NRF1antibody was used at a 5 ug/ml to detect NRF1 in a variety of tissues.? This image shows NRF1 staining of human testis.? The antibody shows nuclear staining in lymphocytes. Tissue was formalin-fixed and paraffin embedded.? Personal Communication, Tina Roush, LifeSpanBiosciences, Seattle, WA.
- Validation comment
- IHC
