PAB21395

antibody from Abnova Corporation

Targeting: IGF2BP1 IMP-1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB21395

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB21395, RRID:AB_10963108

Product name

IGF2BP1 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant IGF2BP1.

Antigen sequence

DVAAMSLQSHLIPGLNLAAVGLFPASSSAVPPPPS
SVTGAAPYSSFMQAPEQEMVQVFIPAQAVGAIIGK
K


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: Human Plasma, Lane 4: Liver, Lane 5: Tonsil with IGF2BP1 polyclonal antibody (Cat # PAB21395) at 1:250-1:500 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line A-431 with IGF2BP1 polyclonal antibody (Cat # PAB21395) at 1-4 ug/mL dilution shows positivity in nucleus but not nucleoli, plasma membrane, cytoplasm and vesicles.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human colon with IGF2BP1 polyclonal antibody (Cat # PAB21395) shows distinct positivity in cytoplsma and nuclei of glandular cells at 1:50-1:200 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)