PAB21413

antibody from Abnova Corporation

Targeting: SMAP2 SMAP1L

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB21413

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB21413, RRID:AB_10966211

Product name

SMAP2 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant SMAP2.

Antigen sequence

ASGMVAPMAMPAGYMGGMQASMMGVPNGMMTTQQA
GYMAGMAAMPQTVYGVQPAQQLQWNLTQMTQQMAG
MNFYGANGMMNYGQSMSGGNGQAANQTLSPQMWK


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: Human Plasma, Lane 4: Liver, Lane 5: Tonsil with SMAP2 polyclonal antibody (Cat # PAB21413) at 1:250-1:500 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line A-431 with SMAP2 polyclonal antibody (Cat # PAB21413) at 1-4 ug/mL dilution shows positivity in nucleus but not nucleoli and cytoplasm.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human lymph node with SMAP2 polyclonal antibody (Cat # PAB21413) shows strong positivity in cells outside the germinal center at 1:200-1:500 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)