11913-1-AP

antibody from Invitrogen Antibodies

Targeting: EXOC4 KIAA1699, MGC27170, SEC8, SEC8L1, Sec8p

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

11913-1-AP

Provider

Invitrogen Antibodies

Product name

Sec8 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: KHVLNLLDE IENIKQVPQK LEQCMASKHY LSATDMLVSA VESLEGPLLQ VEGLSDLRLE LHSKKMNLHL VLIDELHRHL YIKSTSRVVQ RNKEKGKISS LVKDASVPLI DVTNLPTPRK FLDTSHYSTA GSSSVREINL QDIKEDLELD PEENSTLFMG ILIKGLAKLK KIPETVKAII ERLEQELKQI VKRSTTQVAD SGYQRGENVT VENQPRLLLE LLELLFDKFN AVAAAHSVVL GYLQDTVVTP LTQQEDIKLY DMADVWVKIQ DVLQMLLTEY LDMKNTRTAS EPSAQLSYAS TGREFAAFFA KKKPQRPKNS LFKFESSSHA ISMSAYLREQ RRELYSRSGE LQGE (122-474 aa encoded by B C026174)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.15 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Jurkat cells were subjected to SDS PAGE followed by western blot with 11913-1-AP (Sec8 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse testis tissue were subjected to SDS PAGE followed by western blot with 11913-1-AP (Sec8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

U-251 cells were subjected to SDS PAGE followed by western blot with 11913-1-AP (Sec8 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human colon cancer using 11913-1-AP (Sec8 antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using (Product # 11913-1-AP) (Sec8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-Sec8 (IP:11913-1-AP, 4ug; Detection:11913-1-AP 1:300) with Jurkat cells lysate 2800ug.