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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [4]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Other assay [2]
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- Product number
- PA5-27207 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IGF1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: PC-3, U87-MG, SK-N-SH.
- Concentration
- 1.16 mg/mL
Submitted references Translation of Tudor-SN, a novel terminal oligo-pyrimidine (TOP) mRNA, is regulated by the mTORC1 pathway in cardiomyocytes.
Innovative Three-Dimensional Microscopic Analysis of Uremic Growth Plate Discloses Alterations in the Process of Chondrocyte Hypertrophy: Effects of Growth Hormone Treatment.
The role of miR-24 as a race related genetic factor in prostate cancer.
Differential expression of miR-34b and androgen receptor pathway regulate prostate cancer aggressiveness between African-Americans and Caucasians.
Gan S, Su C, Ma J, Liu M, Cui X, Xin L, Ren Y, Gao X, Ge L, Wei M, Yang J
RNA biology 2021 Jun;18(6):900-913
RNA biology 2021 Jun;18(6):900-913
Innovative Three-Dimensional Microscopic Analysis of Uremic Growth Plate Discloses Alterations in the Process of Chondrocyte Hypertrophy: Effects of Growth Hormone Treatment.
Fernández-Iglesias Á, Fuente R, Gil-Peña H, Alonso-Durán L, García-Bengoa M, Santos F, López JM
International journal of molecular sciences 2020 Jun 25;21(12)
International journal of molecular sciences 2020 Jun 25;21(12)
The role of miR-24 as a race related genetic factor in prostate cancer.
Hashimoto Y, Shiina M, Kato T, Yamamura S, Tanaka Y, Majid S, Saini S, Shahryari V, Kulkarni P, Dasgupta P, Mitsui Y, Sumida M, Deng G, Tabatabai L, Kumar D, Dahiya R
Oncotarget 2017 Mar 7;8(10):16581-16593
Oncotarget 2017 Mar 7;8(10):16581-16593
Differential expression of miR-34b and androgen receptor pathway regulate prostate cancer aggressiveness between African-Americans and Caucasians.
Shiina M, Hashimoto Y, Kato T, Yamamura S, Tanaka Y, Majid S, Saini S, Varahram S, Kulkarni P, Dasgupta P, Mitsui Y, Sumida M, Tabatabai L, Deng G, Kumar D, Dahiya R
Oncotarget 2017 Jan 31;8(5):8356-8368
Oncotarget 2017 Jan 31;8(5):8356-8368
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of IGF1 using 30 µg of A) PC-3 (B) U87-MG and C) SK-N-SH lysate. Samples were loaded onto a 15% SDS-PAGE gel and probed with an IGF1 polyclonal antibody (Product # PA5-27207) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using IGF1 Polyclonal Antibody (Product # PA5-27207). Various whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with IGF1 Polyclonal Antibody (Product # PA5-27207) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IGF1 Polyclonal Antibody detects IGF1 protein at Golgi apparatus membrane by immunofluorescent analysis. Sample: SKNSH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: IGF1 protein stained by IGF1 Polyclonal Antibody (Product # PA5-27207) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IGF1 Polyclonal Antibody detects IGF1 protein at cytoplasm on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. IGF1 Polyclonal Antibody (Product # PA5-27207) diluted at 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 4 miR-34b target genes and luciferase reporter assay A. qPCR analysis of AR, BCL2, ETV1, PDPK1 and IGF1 from MDA-PCa-2b or DU-145 cells transfected with miR-34b-3p mimic. Asterisks indicate a p-value
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 5 Predicted target gene expression changes after miR-24 induction ( A ) qPCR analyses for the predicted target genes of miR-24. We checked candidate gene expression levels after miR-24 over-expression. * p < 0.05, Bar = +- SEM. ( B ) Western blotting of miR-24 target genes. ( C )The results of Dual Luciferase Reporter Assays. Except for IGF1, all genes have two different binding sites (for instance; AR-1 and AR-2 mean that miR-24 was predicted that it may bind to two different site of AR mRNA). * p < 0.05, n.s; no significance, Bar = +- SEM. ( D ) Linear regression model of mRNA expression levels between miR-24-1 and its five target genes in human PCa samples.
