PA5-78083

antibody from Invitrogen Antibodies

Targeting: NPAS1 bHLHe11, MOP5, PASD5

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-78083

Provider

Invitrogen Antibodies

Product name

NPAS1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Positive Control: U87-MG, IMR32, SK-N-AS

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NPAS1 Polyclonal Antibody detects NPAS1 protein by Western blot analysis. A. 30 µg U87-MG whole cell lysate/extract. B. 30 µg IMR32 whole cell lysate/extract. C. 30 µg SK-N-AS whole cell lysate/extract.7.5 % SDS-PAGE. NPAS1 Polyclonal Antibody (Product # PA5-78083) dilution: 1:1,000.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NPAS1 Polyclonal Antibody detects NPAS1 protein at nucleus by immunofluorescent analysis. Sample: U87-MG cells were fixed in 4% paraformaldehyde/PBS for 15 min. Green: NPAS1 protein stained by NPAS1 Polyclonal Antibody (Product # PA5-78083) diluted at 1:500. Blue: Hoechst 33342 staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NPAS1 Polyclonal Antibody detects NPAS1 protein at nucleus by immunofluorescent analysis. Sample: U87-MG cells were fixed in 4% paraformaldehyde/PBS for 15 min. Green: NPAS1 protein stained by NPAS1 Polyclonal Antibody (Product # PA5-78083) diluted at 1:500. Blue: Hoechst 33342 staining.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NPAS1 Polyclonal Antibody detects NPAS1 protein at nucleus on mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. NPAS1 Polyclonal Antibody (Product # PA5-78083) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NPAS1 Polyclonal Antibody detects NPAS1 protein at nucleus on mouse middle brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse middle brain. NPAS1 Polyclonal Antibody (Product # PA5-78083) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NPAS1 Polyclonal Antibody detects NPAS1 protein at nucleus on rat fore brain by immunohistochemical analysis. Sample: Paraffin-embedded rat fore brain. NPAS1 Polyclonal Antibody (Product # PA5-78083) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.