PAB23337

antibody from Abnova Corporation

Targeting: ALKBH8 MGC10235, TRM9, TRMT9A

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB23337

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB23337, RRID:AB_11121376

Product name

ALKBH8 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant ALKBH8.

Antigen sequence

LPPGLMVVEEIISSEEEKMLLESVDWTEDTDNQNS
QKSLKHRRVKHFGYEFHYENNNVDKDKPLSGGLPD
ICESFLEKWLRKGYIKHKPDQMTINQYEPGQG


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: Human Plasma, Lane 4: Liver, Lane 5: Tonsil with ALKBH8 polyclonal antibody (Cat # PAB23337) at 1:250-1:500 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line A-431 with ALKBH8 polyclonal antibody (Cat # PAB23337) at 1-4 ug/mL dilution shows positivity in nucleus but not nucleoli and cytoplasm.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human small intestine with ALKBH8 polyclonal antibody (Cat # PAB23337) shows strong membranous positivity in glandular cells at 1:200-1:500 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)