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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
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- Product number
- PA5-28204 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Carboxypeptidase B1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: AsPC-1, mouse pancreas, rat pancreas.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Carboxypeptidase B using 30 µg of A) HeLa S3 and B) HepG2 lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a Carboxypeptidase B polyclonal antibody (Product # PA5-28204) at a dilution of 1:200.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Carboxypeptidase B1 was performed by separating 50 µg of mouse tissue extract by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a Carboxypeptidase B1 Polyclonal Antibody (Product # PA5-28204) at a dilution of 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Carboxypeptidase B1 was performed by separating 50 µg of rat tissue extract by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a Carboxypeptidase B1 Polyclonal Antibody (Product # PA5-28204) at a dilution of 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of Carboxypeptidase B1 was performed by separating 30 µg of various whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a Carboxypeptidase B1 Polyclonal Antibody (Product # PA5-28204) at a dilution of 1:500.
