PA5-28625

antibody from Invitrogen Antibodies

Targeting: SART3 KIAA0156, p110, RP11-13G14, TIP110

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-28625

Provider

Invitrogen Antibodies

Product name

SART3 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Recommended positive controls: A431, H1299, HeLa, HepG2, Raji.

Concentration

0.52 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using SART3 Polyclonal Antibody (Product # PA5-28625). Sample (30 µg of whole cell lysate). Lane A: A431. Lane B: H1299. Lane C: Hela . Lane D: Hep G2 . 7.5% SDS PAGE. SART3 Polyclonal Antibody (Product # PA5-28625) diluted at 1:1,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using SART3 Polyclonal Antibody (Product # PA5-28625). Sample (30 µg of whole cell lysate). Lane A: A431. Lane B: H1299. Lane C: Hela . Lane D: Hep G2 . 7.5% SDS PAGE. SART3 Polyclonal Antibody (Product # PA5-28625) diluted at 1:1,000.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of SART3 in paraformaldehyde-fixed HeLa cells using a SART3 polyclonal antibody (Product # PA5-28625) at a 1:200 dilution.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded NCI-N87 xenograft, using SART3 (Product # PA5-28625) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

SART3 Polyclonal Antibody immunoprecipitates SART3 protein in IP experiments. IP samples: HepG2 whole cell extract. A. Control with 4 µg of preimmune Rabbit IgG. B. Immunoprecipitation of SART3 protein by 4 µg SART3 Polyclonal Antibody (Product # PA5-28625). 5 % SDS-PAGE. The immunoprecipitated SART3 protein was detected by SART3 Polyclonal Antibody (Product # PA5-28625) diluted at 1:2,000.