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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [2]
- ELISA [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
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- Product number
- H00002512-M18 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00002512-M18, RRID:AB_11040871
- Product name
- Mouse Monoclonal Ferritin Light Chain Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Ferritin Light Chain Antibody (X3) [H00002512-M18] - Analysis of FTL over-expressed 293 cell line, cotransfected with FTL Validated Chimera RNAi (Cat # H00002512-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with FTL monoclonal antibody (M18), clone X3 (Cat # H00002512-M18). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Ferritin Light Chain Antibody (X3) [H00002512-M18] - Analysis of FTL expression in transfected 293T cell line by FTL monoclonal antibody (M18), clone X3. Lane 1: FTL transfected lysatE (20 KDa). Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: Ferritin Light Chain Antibody (X3) [H00002512-M18] - Detection limit for recombinant GST tagged FTL is approximately 0.03ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: Ferritin Light Chain Antibody (X3) [H00002512-M18] - Analysis of monoclonal antibody to FTL on HeLa cell. Antibody concentration 10 ug/ml
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: Ferritin Light Chain Antibody (X3) [H00002512-M18] - Analysis of FTL transfected lysate using anti-FTL monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with FTL rabbit polyclonal antibody.
