11744-1-AP

antibody from Proteintech Group

Targeting: IFT81 CDV-1R, CDV1, MGC4027

Provider product page for 11744-1-AP

Western blot

ELISA

Immunocytochemistry

Immunoprecipitation

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [32]
Comments [0]
Validations
Western blot [1]
Immunoprecipitation [1]
Immunohistochemistry [1]

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Product number: 11744-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#11744-1-AP, RRID:AB_2121966
Product name: IFT81 antibody
Antibody type: Polyclonal
Description: KD/KO validated IFT81 antibody (Cat. #11744-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, Canine and has been validated for the following applications: IF, IHC, IP, WB,ELISA.
Reactivity: Human, Mouse, Canine
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

IFT81 protein structure - 11744-1-AP shown in red.

Submitted references Cilia locally synthesize proteins to sustain their ultrastructure and functions.

Hao K, Chen Y, Yan X, Zhu X
Nature communications 2021 Nov 30;12(1):6971

Separable roles for RanGTP in nuclear and ciliary trafficking of a kinesin-2 subunit.

Huang S, Dougherty LL, Avasthi P
The Journal of biological chemistry 2021 Jan-Jun;296:100117

Mutations in GRK2 cause Jeune syndrome by impairing Hedgehog and canonical Wnt signaling.

Bosakova M, Abraham SP, Nita A, Hruba E, Buchtova M, Taylor SP, Duran I, Martin J, Svozilova K, Barta T, Varecha M, Balek L, Kohoutek J, Radaszkiewicz T, Pusapati GV, Bryja V, Rush ET, Thiffault I, Nickerson DA, Bamshad MJ, University of Washington Center for Mendelian Genomics., Rohatgi R, Cohn DH, Krakow D, Krejci P
EMBO molecular medicine 2020 Nov 6;12(11):e11739

Genetic interaction of mammalian IFT-A paralogs regulates cilia disassembly, ciliary entry of membrane protein, Hedgehog signaling, and embryogenesis.

Wang W, Allard BA, Pottorf TS, Wang HH, Vivian JL, Tran PV
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2020 May;34(5):6369-6381

IFT proteins interact with HSET to promote supernumerary centrosome clustering in mitosis.

Vitre B, Taulet N, Guesdon A, Douanier A, Dosdane A, Cisneros M, Maurin J, Hettinger S, Anguille C, Taschner M, Lorentzen E, Delaval B
EMBO reports 2020 Jun 4;21(6):e49234

The essential role of intraflagellar transport protein IFT81 in male mice spermiogenesis and fertility.

Qu W, Yuan S, Quan C, Huang Q, Zhou Q, Yap Y, Shi L, Zhang D, Guest T, Li W, Yee SP, Zhang L, Cazin C, Hess RA, Ray PF, Kherraf ZE, Zhang Z
American journal of physiology. Cell physiology 2020 Jun 1;318(6):C1092-C1106

Intraflagellar-transport A dysfunction causes hyperphagia-induced systemic insulin resistance in a pre-obese state.

Jacobs DT, Allard BA, Pottorf TS, Silva LM, Wang W, Al-Naamani A, Agborbesong E, Wang T, Carr DA, Tran PV
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2020 Jan;34(1):148-160

The novel ciliogenesis regulator DYRK2 governs Hedgehog signaling during mouse embryogenesis.

Yoshida S, Aoki K, Fujiwara K, Nakakura T, Kawamura A, Yamada K, Ono M, Yogosawa S, Yoshida K
eLife 2020 Aug 6;9

Murine germ cell-specific disruption of Ift172 causes defects in spermiogenesis and male fertility.

Zhang S, Liu Y, Huang Q, Yuan S, Liu H, Shi L, Yap YT, Li W, Zhen J, Zhang L, Hess RA, Zhang Z
Reproduction (Cambridge, England) 2020 Apr;159(4):409-421

Intraflagellar transport protein 74 is essential for spermatogenesis and male fertility in mice†.

Shi L, Zhou T, Huang Q, Zhang S, Li W, Zhang L, Hess RA, Pazour GJ, Zhang Z
Biology of reproduction 2019 Jul 1;101(1):188-199

Inhibition of Hedgehog signaling suppresses proliferation and microcyst formation of human Autosomal Dominant Polycystic Kidney Disease cells.

Silva LM, Jacobs DT, Allard BA, Fields TA, Sharma M, Wallace DP, Tran PV
Scientific reports 2018 Mar 21;8(1):4985

Primary Cilia Mediate Diverse Kinase Inhibitor Resistance Mechanisms in Cancer.

Jenks AD, Vyse S, Wong JP, Kostaras E, Keller D, Burgoyne T, Shoemark A, Tsalikis A, de la Roche M, Michaelis M, Cinatl J Jr, Huang PH, Tanos BE
Cell reports 2018 Jun 5;23(10):3042-3055

The small GTPase RSG1 controls a final step in primary cilia initiation.

Agbu SO, Liang Y, Liu A, Anderson KV
The Journal of cell biology 2018 Jan 2;217(1):413-427

Intraflagellar transporter protein 140 (IFT140), a component of IFT-A complex, is essential for male fertility and spermiogenesis in mice.

Zhang Y, Liu H, Li W, Zhang Z, Zhang S, Teves ME, Stevens C, Foster JA, Campbell GE, Windle JJ, Hess RA, Pazour GJ, Zhang Z
Cytoskeleton (Hoboken, N.J.) 2018 Feb;75(2):70-84

Spinocerebellar ataxia type 11-associated alleles of Ttbk2 dominantly interfere with ciliogenesis and cilium stability.

Bowie E, Norris R, Anderson KV, Goetz SC
PLoS genetics 2018 Dec;14(12):e1007844

The Meckel syndrome- associated protein MKS1 functionally interacts with components of the BBSome and IFT complexes to mediate ciliary trafficking and hedgehog signaling.

Goetz SC, Bangs F, Barrington CL, Katsanis N, Anderson KV
PloS one 2017;12(3):e0173399

Palmitoylation of the ciliary GTPase ARL13b is necessary for its stability and its role in cilia formation.

Roy K, Jerman S, Jozsef L, McNamara T, Onyekaba G, Sun Z, Marin EP
The Journal of biological chemistry 2017 Oct 27;292(43):17703-17717

IFT25, an intraflagellar transporter protein dispensable for ciliogenesis in somatic cells, is essential for sperm flagella formation.

Liu H, Li W, Zhang Y, Zhang Z, Shang X, Zhang L, Zhang S, Li Y, Somoza AV, Delpi B, Gerton GL, Foster JA, Hess RA, Pazour GJ, Zhang Z
Biology of reproduction 2017 May 1;96(5):993-1006

The CEP19-RABL2 GTPase Complex Binds IFT-B to Initiate Intraflagellar Transport at the Ciliary Base.

Kanie T, Abbott KL, Mooney NA, Plowey ED, Demeter J, Jackson PK
Developmental cell 2017 Jul 10;42(1):22-36.e12

Dynamic Remodeling of Membrane Composition Drives Cell Cycle through Primary Cilia Excision.

Phua SC, Chiba S, Suzuki M, Su E, Roberson EC, Pusapati GV, Schurmans S, Setou M, Rohatgi R, Reiter JF, Ikegami K, Inoue T
Cell 2017 Jan 12;168(1-2):264-279.e15

IFT proteins spatially control the geometry of cleavage furrow ingression and lumen positioning.

Taulet N, Vitre B, Anguille C, Douanier A, Rocancourt M, Taschner M, Lorentzen E, Echard A, Delaval B
Nature communications 2017 Dec 4;8(1):1928

Intraflagellar transporter protein (IFT27), an IFT25 binding partner, is essential for male fertility and spermiogenesis in mice.

Zhang Y, Liu H, Li W, Zhang Z, Shang X, Zhang D, Li Y, Zhang S, Liu J, Hess RA, Pazour GJ, Zhang Z
Developmental biology 2017 Dec 1;432(1):125-139

KDM3A coordinates actin dynamics with intraflagellar transport to regulate cilia stability.

Yeyati PL, Schiller R, Mali G, Kasioulis I, Kawamura A, Adams IR, Playfoot C, Gilbert N, van Heyningen V, Wills J, von Kriegsheim A, Finch A, Sakai J, Schofield CJ, Jackson IJ, Mill P
The Journal of cell biology 2017 Apr 3;216(4):999-1013

IFT56 regulates vertebrate developmental patterning by maintaining IFTB complex integrity and ciliary microtubule architecture.

Xin D, Christopher KJ, Zeng L, Kong Y, Weatherbee SD
Development (Cambridge, England) 2017 Apr 15;144(8):1544-1553

Immunofluorescent staining of septins in primary cilia.

Kim MS, Froese CD, Xie H, Trimble WS
Methods in cell biology 2016;136:269-83

Destabilization of the IFT-B cilia core complex due to mutations in IFT81 causes a Spectrum of Short-Rib Polydactyly Syndrome.

Duran I, Taylor SP, Zhang W, Martin J, Forlenza KN, Spiro RP, Nickerson DA, Bamshad M, Cohn DH, Krakow D
Scientific reports 2016 Sep 26;6:34232

IFT52 mutations destabilize anterograde complex assembly, disrupt ciliogenesis and result in short rib polydactyly syndrome.

Zhang W, Taylor SP, Nevarez L, Lachman RS, Nickerson DA, Bamshad M, University of Washington Center for Mendelian Genomics Consortium., Krakow D, Cohn DH
Human molecular genetics 2016 Sep 15;25(18):4012-4020

Novel role for the midbody in primary ciliogenesis by polarized epithelial cells.

Bernabé-Rubio M, Andrés G, Casares-Arias J, Fernández-Barrera J, Rangel L, Reglero-Real N, Gershlick DC, Fernández JJ, Millán J, Correas I, Miguez DG, Alonso MA
The Journal of cell biology 2016 Aug 1;214(3):259-73

IFT81, encoding an IFT-B core protein, as a very rare cause of a ciliopathy phenotype.

Perrault I, Halbritter J, Porath JD, Gérard X, Braun DA, Gee HY, Fathy HM, Saunier S, Cormier-Daire V, Thomas S, Attié-Bitach T, Boddaert N, Taschner M, Schueler M, Lorentzen E, Lifton RP, Lawson JA, Garfa-Traore M, Otto EA, Bastin P, Caillaud C, Kaplan J, Rozet JM, Hildebrandt F
Journal of medical genetics 2015 Oct;52(10):657-65

Systematic proteomics of the VCP-UBXD adaptor network identifies a role for UBXN10 in regulating ciliogenesis.

Raman M, Sergeev M, Garnaas M, Lydeard JR, Huttlin EL, Goessling W, Shah JV, Harper JW
Nature cell biology 2015 Oct;17(10):1356-69

The kinesin-4 protein Kif7 regulates mammalian Hedgehog signalling by organizing the cilium tip compartment.

He M, Subramanian R, Bangs F, Omelchenko T, Liem KF Jr, Kapoor TM, Anderson KV
Nature cell biology 2014 Jul;16(7):663-72

BBS proteins interact genetically with the IFT pathway to influence SHH-related phenotypes.

Zhang Q, Seo S, Bugge K, Stone EM, Sheffield VC
Human molecular genetics 2012 May 1;21(9):1945-53

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: human brain tissue were subjected to SDS PAGE followed by western blot with 11744-1-AP(IFT81 antibody) at dilution of 1:600
Sample type: tissue

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: IP Result of anti-IFT81 (IP:11744-1-AP, 3ug; Detection:11744-1-AP 1:500) with mouse brain tissue lysate 7500ug.
Sample type: tissue

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human prostate cancer using 11744-1-AP(IFT81 antibody) at dilution of 1:50 (under 10x lens)
Sample type: tissue