Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- GTX111938 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX111938, RRID:AB_11162308
- Product name
- Securin antibody
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
No comments: Submit comment
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: IMR32 12% SDS PAGE GTX111938 diluted at 1:1000
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Securin antibody detects PTTG1 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: MCF-7 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: PTTG1 protein stained by Securin antibody (GTX111938) diluted at 1:500.Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Securin antibody immunoprecipitates Securin protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A. Control with 2 £gg of preimmune rabbit IgG B. Immunoprecipitation of Securin protein by 2 £gg of Securin antibody (GTX111938) 12% SDS-PAGE The immunoprecipitated Securin protein was detected by Securin antibody (GTX111938) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Securin antibody detects PTTG1 protein at on BT483 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded BT483 xenograft. Securin antibody (GTX111938) dilution: 1:500.
