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LearnGTX114210
antibody from GeneTex
Targeting: TARDBP
ALS10, TDP-43
Western blot Immunocytochemistry Immunoprecipitation Immunohistochemistry
Chromatin ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [7]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [2]
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- Product number
- GTX114210 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX114210, RRID:AB_2038095
- Product name
- TDP43 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
Submitted references Co-regulation of mRNA translation by TDP-43 and Fragile X Syndrome protein FMRP.
Resources for the Comprehensive Discovery of Functional RNA Elements.
Metabolism and mis-metabolism of the neuropathological signature protein TDP-43.
Majumder P, Chu JF, Chatterjee B, Swamy KB, Shen CJ
Acta neuropathologica 2016 Nov;132(5):721-738
Acta neuropathologica 2016 Nov;132(5):721-738
Resources for the Comprehensive Discovery of Functional RNA Elements.
Sundararaman B, Zhan L, Blue SM, Stanton R, Elkins K, Olson S, Wei X, Van Nostrand EL, Pratt GA, Huelga SC, Smalec BM, Wang X, Hong EL, Davidson JM, Lécuyer E, Graveley BR, Yeo GW
Molecular cell 2016 Mar 17;61(6):903-13
Molecular cell 2016 Mar 17;61(6):903-13
Metabolism and mis-metabolism of the neuropathological signature protein TDP-43.
Huang CC, Bose JK, Majumder P, Lee KH, Huang JT, Huang JK, Shen CK
Journal of cell science 2014 Jul 15;127(Pt 14):3024-38
Journal of cell science 2014 Jul 15;127(Pt 14):3024-38
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- TARDBP antibody detects TARDBP protein by western blot analysis.A. 30 ?g Neuro2A whole cell lysate/extractB. 30 ?g GL261 whole cell lysate/extractC. 30 ?g NIH-3T3 whole cell lysate/extractD. 30 ?g BCL-1 whole cell lysate/extractE. 30 ?g Raw264.7 whole cell lysate/extract10% SDS-PAGETARDBP antibody (GTX114210) dilution: 1:3000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ?g of whole cell lysate) A: A431 (GTX27909) B: H1299 C: HeLa 10% SDS PAGE GTX114210 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western blot analysis of TDP-43 (GTX114210, upper panel) and beta-actin (GTX109639, lower panel)? Sample (30 ?g of whole cell lysate) ? A: HeLa mock control? B: HeLa transfected shTDP-43 10% SDS PAGE? GTX114210 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (50 ?g of whole cell lysate) A: Rat brain 10% SDS PAGE GTX114210 diluted at 1:3000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western blot analysis of TDP-43 (GTX114210, upper panel) and beta-actin (GTX109639, lower panel)? Sample (30 ?g of whole cell lysate) ? A: HeLa mock control? B: HeLa transfected shTDP-43 10% SDS PAGE? GTX114210 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with TDP43 antibody (GTX114210) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with TDP43 antibody (GTX114210) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using TDP-43(GTX114210) antibody at 1:200 dilution.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- TARDBP antibody immunoprecipitates TARDBP protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A. 40 £gg HeLa whole cell lysate/extract B. Control with 2 £gg of preimmune rabbit IgG C. Immunoprecipitation of TARDBP protein by 2 £gg of TARDBP antibody (GTX114210) 12% SDS-PAGE The immunoprecipitated TARDBP protein was detected by TARDBP antibody (GTX114210) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded rat brain, using TARDBP(GTX114210) antibody at 1:500 dilution.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded Cal27 Xenograft , using TDP-43(GTX114210) antibody at 1:100 dilution.
