MAB2040

antibody from Abnova Corporation

Targeting: TARDBP ALS10, TDP-43

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB2040

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#MAB2040, RRID:AB_1715468

Product name

TARDBP monoclonal antibody, clone k1B8

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against partial recombinant TARDBP.

Isotype

IgG

Antibody clone number

k1B8

Storage

Store at -20°C.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

SOD1 protein aggregates stimulate macropinocytosis in neurons to facilitate their propagation.

Zeineddine R, Pundavela JF, Corcoran L, Stewart EM, Do-Ha D, Bax M, Guillemin G, Vine KL, Hatters DM, Ecroyd H, Dobson CM, Turner BJ, Ooi L, Wilson MR, Cashman NR, Yerbury JJ
Molecular neurodegeneration 2015 Oct 31;10:57

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Novel mutations in TARDBP (TDP-43) in patients with familial amyotrophic lateral sclerosis.

Rutherford NJ, Zhang YJ, Baker M, Gass JM, Finch NA, Xu YF, Stewart H, Kelley BJ, Kuntz K, Crook RJ, Sreedharan J, Vance C, Sorenson E, Lippa C, Bigio EH, Geschwind DH, Knopman DS, Mitsumoto H, Petersen RC, Cashman NR, Hutton M, Shaw CE, Boylan KB, Boeve B, Graff-Radford NR, Wszolek ZK, Caselli RJ, Dickson DW, Mackenzie IR, Petrucelli L, Rademakers R
PLoS genetics 2008 Sep 19;4(9):e1000193

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TARDBP mutations in individuals with sporadic and familial amyotrophic lateral sclerosis.

Kabashi E, Valdmanis PN, Dion P, Spiegelman D, McConkey BJ, Vande Velde C, Bouchard JP, Lacomblez L, Pochigaeva K, Salachas F, Pradat PF, Camu W, Meininger V, Dupre N, Rouleau GA
Nature genetics 2008 May;40(5):572-4

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Cloning and characterization of a novel cellular protein, TDP-43, that binds to human immunodeficiency virus type 1 TAR DNA sequence motifs.

Ou SH, Wu F, Harrich D, García-Martínez LF, Gaynor RB
Journal of virology 1995 Jun;69(6):3584-96

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Cell lysates of HeLa (20 ug) were resolved by SDS-PAGE and probed with TARDBP monoclonal antibody, clone k1B8 (Cat # MAB2040) (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Paraffin embedded sections of human colon cancer tissue were incubated with TARDBP monoclonal antibody, clone k1B8 (Cat # MAB2040) (1:100).

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)