10782-2-AP

antibody from Invitrogen Antibodies

Targeting: TARDBP ALS10, TDP-43

Provider product page for 10782-2-AP

Western blot

Immunocytochemistry

Immunoprecipitation

Immunohistochemistry

Flow cytometry

Chromatin Immunoprecipitation

Other assay

Antibody data

Antibody Data
Antigen structure
References [0]
Comments [0]
Validations
Western blot [8]
Immunocytochemistry [5]
Immunohistochemistry [14]
Flow cytometry [1]
Other assay [2]

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Product number: 10782-2-AP - Provider product page
Provider: Invitrogen Antibodies
Product name: TDP-43 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Other
Description: Proteintech's 10782-2-AP antibody is a rabbit polyclonal antibody recognizing N-terminal TDP-43. It recognizes the intact 45 kDa protein as well as all post translationally modified and truncated forms in multiple applications. Various forms of TDP-43 exist, including 18-35 kDa of cleaved C-terminal fragments, 45-50 kDa phospho-protein, 55 kDa glycosylated form, 75 kDa hyperphosphorylated form, and 90-300 kDa cross-linked form. Recently, TDP-43 has been reported to be overexpressed in triple negative breast cancer (TNBC) and it may be a potential target for TNBC diagnosis and drug design.
Reactivity: Human, Mouse, Rat, Zebrafish
Host: Rabbit
Isotype: IgG
Vial size: 150 µL
Concentration: 0.31 mg/mL
Storage: -20°C

TARDBP protein structure - 10782-2-AP shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: HeLa cells were subjected to SDS PAGE followed by western blot with 10782-2-AP (TARDBP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 10782-2-AP (TARDBP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: A549 cells (shcontrol and shRNA of TDP43) were subjected to SDS PAGE followed by western blot with 10782-2-AP (TARDBP antibody) at dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Human heart tissue were subjected to SDS PAGE followed by western blot with 10782-2-AP (TARDBP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: HL-60 cells were subjected to SDS PAGE followed by western blot with 10782-2-AP (TARDBP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: K-562 cells were subjected to SDS PAGE followed by western blot with 10782-2-AP (TARDBP antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Mouse brain tissue were subjected to SDS PAGE followed by western blot with 10782-2-AP (TDP-43 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Rat brain tissue were subjected to SDS PAGE followed by western blot with 10782-2-AP (TDP-43 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 10782-2-AP (TDP-43 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 10782-2-AP (TDP-43 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry of paraffin-embedded human brain using 10782-2-AP (TARDBP antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry of paraffin-embedded human gliomas tissue slide using 10782-2-AP (TDP-43 Antibody) at dilution of 1:2000 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry of paraffin-embedded human brain using 10782-2-AP (TARDBP antibody) at dilution of 1:50 (under 40x lens).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry of paraffin-embedded human pancreas tissue slide using 10782-2-AP (TDP-43 Antibody) at dilution of 1:200 (under 10x lens).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry of paraffin-embedded human pancreas tissue slide using 10782-2-AP (TDP-43 Antibody) at dilution of 1:200 (under 40x lens).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry of paraffin-embedded mouse brain tissue slide using 10782-2-AP (TDP-43 Antibody) at dilution of 1:200 (under 10x lens).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry of paraffin-embedded mouse brain tissue slide using 10782-2-AP (TDP-43 Antibody) at dilution of 1:200 (under 40x lens).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry of paraffin-embedded rat brain tissue slide using 10782-2-AP (TDP-43 Antibody) at dilution of 1:200 (under 10x lens).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry of paraffin-embedded rat brain tissue slide using 10782-2-AP (TDP-43 Antibody) at dilution of 1:200 (under 40x lens).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry of paraffin-embedded human prostate cancer using 10782-2-AP (TARDBP antibody) at dilution of 1:100 (under 10x lens).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: 40X of FTLD-U case stained by 10782-2-AP and 60019-2-IG, showing dystrophic neurites. (Figs were provided by Linda K. Kwong).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry of paraffin-embedded human gliomas tissue slide using 10782-2-AP ( TDP-43 Antibody) at dilution of 1:2000 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).