Explore
Validate
Learn
Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-90085 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SWAP70 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: HKEARQRRKE LRKKQLAEQE ELERQMKELQ AANESKQQEL EAVRKKLEEA ASRAAEEEKK RLQTQVELQA RFSTELEREK LIRQQMEEQV AQKSSELEQY LQRVRELEDM YLKLQEALED ERQARQDEET VRKLQARLLE EESSKRAELE KWHLEQQQAI QTTEAEKQEL ENQRVLKEQA LQEAMEQLEQ LELERKQALE QYEEVKKKLE MATNKTKSWK DKVAHHEGLI RLIEPGSKNP HLITNWGPAA FTEAELEERE KNWKEKKTTE; Positive Samples: 293T, A549, LO2; Cellular Location: Cell membrane, Cell projection, Cytoplasm, Nucleus, lamellipodium
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.54 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of extracts of various cell lines, using SWAP70 Polyclonal antibody (Product # PA5-90085) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 5s.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of SWAP70 in extracts of various cell lines using SWAP70 Polyclonal Antibody (Product # PA5-90085) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of SWAP70 was performed in L929 cells using SWAP70 Polyclonal Antibody (Product # PA5-90085) at a dilution of 1:100. Blue: DAPI for nuclear staining.
