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- References [3]
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- Western blot [5]
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- Product number
- GTX70145 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX70145, RRID:AB_372136
- Product name
- DNA ligase III antibody [4C11]
- Antibody type
- Monoclonal
- Reactivity
- Human
- Host
- Mouse
- Storage
- Keep as concentrated solution. Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze-thaw cycles.
Submitted references Rational design of human DNA ligase inhibitors that target cellular DNA replication and repair.
Translocation of XRCC1 and DNA ligase IIIalpha from centrosomes to chromosomes in response to DNA damage in mitotic human cells.
DNA ligase III as a candidate component of backup pathways of nonhomologous end joining.
Chen X, Zhong S, Zhu X, Dziegielewska B, Ellenberger T, Wilson GM, MacKerell AD Jr, Tomkinson AE
Cancer research 2008 May 1;68(9):3169-77
Cancer research 2008 May 1;68(9):3169-77
Translocation of XRCC1 and DNA ligase IIIalpha from centrosomes to chromosomes in response to DNA damage in mitotic human cells.
Okano S, Lan L, Tomkinson AE, Yasui A
Nucleic acids research 2005;33(1):422-9
Nucleic acids research 2005;33(1):422-9
DNA ligase III as a candidate component of backup pathways of nonhomologous end joining.
Wang H, Rosidi B, Perrault R, Wang M, Zhang L, Windhofer F, Iliakis G
Cancer research 2005 May 15;65(10):4020-30
Cancer research 2005 May 15;65(10):4020-30
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 μg of whole cell lysate) A: MCF7 7.5% SDS PAGE GTX70145 diluted at 1:500
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- DNA ligase III antibody [4C11] detects DNA ligase III protein by Western blot analysis.A. 30 µg 293T whole cell lysate/extractB. 30 µg A431 whole cell lysate/extractC. 30 µg HeLa whole cell lysate/extractD. 30 µg HepG2 whole cell lysate/extract7.5 % SDS-PAGEDNA ligase III antibody [4C11] (GTX70145) dilution: 1:500
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- DNA ligase III antibody [4C11] detects DNA ligase III protein by Western blot analysis.A. 30 μg NT2D1 whole cell lysate/extractB. 30 μg PC-3 whole cell lysate/extract7.5 % SDS-PAGEDNA ligase III antibody [4C11] (GTX70145) dilution: 1:500
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western blot analysis of LIG1 in cell lysate from HeLa cells using GTX70145 at 1:1000 dilution.
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- DNA ligase III antibody [4C11] detects DNA ligase III protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DNA ligase III antibody [4C11] (GTX70145) diluted at 1:500.
