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Immunocytochemistry
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [1]
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- Product number
- GTX10354 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX10354, RRID:AB_381009
- Product name
- ASIC3 antibody
- Antibody type
- Polyclonal
- Reactivity
- Rat
- Host
- Guinea Pig
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Supportive validation
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- GeneTex (provider)
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- Experimental details
- ASIC3 expression compared to other nociceptive ion channels. DRG sections double-labeled for ASIC3 (red) and either P2X3 (green, A) orTRPV1?(green, B); double-labeled cells are yellow or orange. Circle charts show the relative distribution of the three receptors among all DRG neurons. ASIC3 and?P2X3?expression overlapped in only 4% of neurons, while some 35% of ASIC3-positive neurons also express TRPV1. The rare cells positive for both ASIC3 and P2X3 were large (eg. cell at lower left in A). 1406 cells counted for A; 995 for B. C, D, electrophysiological recordings reveal a qualitatively similar expression pattern for currents conforming to ASIC3, P2X3, or TRPV1. The cell that generated the currents in C counts as a co-expressor of ASIC3 and P2X3, fitting into the yellow bin in the circle plot. The cell in D is a co-expressor of ASIC3 and TRPV1. Cells were counted positive if they had at least 0.3 nA of current in response to pH 6.8 (ASIC3+), 50 £gM ATP (P2X3+; transient current only), or 1 £gM capsaicin (TRPV1). 182 cells recorded for C; 169 for D.
