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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [2]
- Immunohistochemistry [6]
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- Product number
- NBP1-80792 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-80792, RRID:AB_11012755
- Product name
- Rabbit Polyclonal SPACA1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human SPACA1 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Case report of apoptosis in testis of four AZFc-deleted patients: increased DNA fragmentation during meiosis, but decreased apoptotic markers in post-meiotic germ cells.
Complete deletion of the AZFb interval from the Y chromosome in an oligozoospermic man.
Streichemberger E, Perrin J, Saias-Magnan J, Karsenty G, Malzac P, Grillo JM, Mitchell MJ, Metzler-Guillemain C
Human reproduction (Oxford, England) 2012 Jul;27(7):1939-45
Human reproduction (Oxford, England) 2012 Jul;27(7):1939-45
Complete deletion of the AZFb interval from the Y chromosome in an oligozoospermic man.
Longepied G, Saut N, Aknin-Seifer I, Levy R, Frances AM, Metzler-Guillemain C, Guichaoua MR, Mitchell MJ
Human reproduction (Oxford, England) 2010 Oct;25(10):2655-63
Human reproduction (Oxford, England) 2010 Oct;25(10):2655-63
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SPACA1 Antibody [NBP1-80792] - Analysis in control (vector only transfected HEK293T lysate) and SPACA1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SPACA1 Antibody [NBP1-80792] - Staining of human cerebral cortex.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SPACA1 Antibody [NBP1-80792] - Staining of human endometrium shows low expression as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SPACA1 Antibody [NBP1-80792] - Staining of human testis shows high expression.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SPACA1 Antibody [NBP1-80792] - Staining in human testis and endometrium tissues using anti-SPACA1 antibody. Corresponding SPACA1 RNA-seq data are presented for the same tissues.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SPACA1 Antibody [NBP1-80792] - Staining of human kidney.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SPACA1 Antibody [NBP1-80792] - Staining of human cerebral cortex, kidney, liver and testis using Anti-SPACA1 antibody NBP1-80792 (A) shows similar protein distribution across tissues to independent antibody NBP2-38506 (B).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SPACA1 Antibody [NBP1-80792] - Staining of human liver.
