AP33455PU-N

antibody from OriGene

Targeting: GFRA1 GDNFR, GDNFRA, GFR-ALPHA-1, RET1L, RETL1, TRNR1

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP33455PU-N

Provider

OriGene

Product name

GDNF Receptor alpha 1 (GFRA1) chicken polyclonal antibody, Aff - Purified

Antibody type

Polyclonal

Description

GDNF Receptor alpha 1 (GFRA1) chicken polyclonal antibody, Aff - Purified

Host

Chicken/Avian

Conjugate

Unconjugated

Epitope

GFRA1

Isotype

IgY

Antibody clone number

NULL

Vial size

100 µg

Concentration

1.0 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western-Blot detection of human GFRa-1, using Chicken polyclonal antibody to human GFRa-1. GFRa-1 was C-terminally His tagged. Protein was expressed by CHO cell culture. 100 ng of purified protein was loaded per lane. Lane 1. Prestained protein ladder (Naxo 8003). Liae 2. hGFRa-1. Primary antibody dilution 1:5000 was used. Rabbit anti-Chicken antibody was used as secondary antibody.

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunofluorescence? detection of human GFRa-1 expressed in U2OS cells. GFRa-1 was visualized using Chicken polyclonal antibody to human GFRa-1 (dilution 1:2000) and Goat anti-chicken IgY - H&L DyLight 550 (dilution 1:200) as secondary antibody. For nuclear staining DAPI was used. ArrayScan VTI platform (Thermo Scientific) was used for image acquisition (10x objective). Composite picture was generated using pseudocolors green for GFRa-1 specific signal and blue for nuclei.

Validation comment

IF