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LearnBAF1865
antibody from R&D Systems
Targeting: GZMB
CCPI, CGL-1, CGL1, CSP-B, CSPB, CTLA1, CTSGL1, HLP, SECT
Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Immunohistochemistry [1]
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- Product number
- BAF1865 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse Granzyme B Biotinylated Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse Granzyme B in ELISAs and Western blots. In sandwich immunoassays, less than 1% cross-reactivity with recombinant human (rh) Granzyme A, rhGranzyme B, recombinant mouse (rm) Granzyme D, rmGranzyme G, and rhGranzyme H is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Biotin
- Antigen sequence
P04187- Isotype
- IgG
- Vial size
- 50 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Novel recombinant DNA vaccine candidates for human respiratory syncytial virus: Preclinical evaluation of immunogenicity and protection efficiency.
Mast cells decrease efficacy of anti-angiogenic therapy by secreting matrix-degrading granzyme B.
Improved HSC reconstitution and protection from inflammatory stress and chemotherapy in mice lacking granzyme B.
Human papillomavirus (HPV) type 16 E7 protein bodies cause tumour regression in mice.
Hematopoietic stem cell function and survival depend on c-Myc and N-Myc activity.
Farrag MA, Amer HM, Öhlschläger P, Hamad ME, Almajhdi FN
Human vaccines & immunotherapeutics 2017 Jul 3;13(7):1586-1597
Human vaccines & immunotherapeutics 2017 Jul 3;13(7):1586-1597
Mast cells decrease efficacy of anti-angiogenic therapy by secreting matrix-degrading granzyme B.
Wroblewski M, Bauer R, Cubas Córdova M, Udonta F, Ben-Batalla I, Legler K, Hauser C, Egberts J, Janning M, Velthaus J, Schulze C, Pantel K, Bokemeyer C, Loges S
Nature communications 2017 Aug 16;8(1):269
Nature communications 2017 Aug 16;8(1):269
Improved HSC reconstitution and protection from inflammatory stress and chemotherapy in mice lacking granzyme B.
Carnevalli LS, Scognamiglio R, Cabezas-Wallscheid N, Rahmig S, Laurenti E, Masuda K, Jöckel L, Kuck A, Sujer S, Polykratis A, Erlacher M, Pasparakis M, Essers MA, Trumpp A
The Journal of experimental medicine 2014 May 5;211(5):769-79
The Journal of experimental medicine 2014 May 5;211(5):769-79
Human papillomavirus (HPV) type 16 E7 protein bodies cause tumour regression in mice.
Whitehead M, Ohlschläger P, Almajhdi FN, Alloza L, Marzábal P, Meyers AE, Hitzeroth II, Rybicki EP
BMC cancer 2014 May 24;14:367
BMC cancer 2014 May 24;14:367
Hematopoietic stem cell function and survival depend on c-Myc and N-Myc activity.
Laurenti E, Varnum-Finney B, Wilson A, Ferrero I, Blanco-Bose WE, Ehninger A, Knoepfler PS, Cheng PF, MacDonald HR, Eisenman RN, Bernstein ID, Trumpp A
Cell stem cell 2008 Dec 4;3(6):611-24
Cell stem cell 2008 Dec 4;3(6):611-24
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Granzyme B in Mouse Spleen. Granzyme B was detected in immersion fixed frozen sections of mouse spleen using Goat Anti-Mouse Granzyme B Biotinylated Antigen Affinity-purified Poly-clonal Antibody (Catalog # BAF1865) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counter-stained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
