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LearnPAB29409
antibody from Abnova Corporation
Targeting: GZMB
CCPI, CGL-1, CGL1, CSP-B, CSPB, CTLA1, CTSGL1, HLP, SECT
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PAB29409 - Provider product page
- Provider
- Abnova Corporation
- Product name
- GZMB polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against recombinant human GZMB.
- Antigen sequence
TRAVQPLRLPSNKAQVKPGQTCSVAGWGQTAPLGK
HSHTLQEVKMTVQEDRKCESDLRHYYDSTIELCVG
DPEIKKTSFKGDSGGPLVCNKVAQGIVSYGRNNGM
PPRACTKVSS- Isotype
- IgG
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate) Lane 2: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells) with GZMB polyclonal antibody (Cat # PAB29409) at 1:100-1:250 dilution.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescent staining of human cell line U-2 OS with GZMB polyclonal antibody (Cat # PAB29409) at 1-4 ug/mL concentration shows positivity in vesicles.
- Validation comment
- Immunofluorescence
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunohistochemical staining of human lymph node with GZMB polyclonal antibody (Cat # PAB29409) shows strong cytoplasmic positivity in a subset of lymphoid cells outside reaction center at 1:50-1:200 dilution.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
