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LearnPA5-13518
antibody from Invitrogen Antibodies
Targeting: GZMB
CCPI, CGL-1, CGL1, CSP-B, CSPB, CTLA1, CTSGL1, HLP, SECT
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Flow cytometry [1]
- Other assay [2]
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- Product number
- PA5-13518 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Granzyme B Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 2 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Immune characterization of metastatic colorectal cancer patients post reovirus administration.
Single Extracellular Vesicle Protein Analysis Using Immuno-Droplet Digital Polymerase Chain Reaction Amplification.
Parakrama R, Fogel E, Chandy C, Augustine T, Coffey M, Tesfa L, Goel S, Maitra R
BMC cancer 2020 Jun 18;20(1):569
BMC cancer 2020 Jun 18;20(1):569
Single Extracellular Vesicle Protein Analysis Using Immuno-Droplet Digital Polymerase Chain Reaction Amplification.
Ko J, Wang Y, Carlson JCT, Marquard A, Gungabeesoon J, Charest A, Weitz D, Pittet MJ, Weissleder R
Advanced biosystems 2020 Dec;4(12):e1900307
Advanced biosystems 2020 Dec;4(12):e1900307
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of GZMB (arrow) using a GZMB polyclonal antibody (Product # PA5-13518) in 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GZMB gene.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis using a GZMB polyclonal antibody (Product # PA5-13518) in mouse stomach lysates (35 µg per lane).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis in formalin-fixed, paraffin-embedded human lymph tissue using a GZMB polyclonal antibody (Product # PA5-13518), followed by HRP-conjugated secondary antibody and DAB staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of HL-60 cells using a GZMB polyclonal antibody (Product # PA5-13518) (right) compared to a negative control cell (left) at a dilution of 1:10-50, followed by a FITC-conjugated goat anti-rabbit antibody
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 3 shows pre- ( a ) and post- ( b ) reovirus IHC staining of the biopsied tumor tissue stained with granzyme B at 40X magnification. Post reovirus (b) section shows atrophy of tumor cells in discrete islands with strong staining for granzyme B and surrounded by fibrous connective tissues a phenomenon commonly observed post tumor regression
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 5 Figure Multiplexed single EV protein profiling. A) Duplexing is developed and tested using EGFR and EpCAM protein profiling. Double positive (EGFR+ EpCAM+) EV subpopulation can be identified through multiplexing. Individual droplets are plotted (gray: empty or specific protein negative droplets, color: specific protein positive droplets). B) Single vesicle multiplexing was expanded to profile three targets (PD-L1, GZMB, and TCF7) from mouse plasma EV using FAM, HEX, and Cy5 fluorophores respectively (scale bar = 100 um).
