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LearnALX-804-121F-T100
antibody from Enzo Life Sciences
Targeting: GZMB
CCPI, CGL-1, CGL1, CSP-B, CSPB, CTLA1, CTSGL1, HLP, SECT
Immunocytochemistry
Immunohistochemistry
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [6]
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- Validations
- Immunocytochemistry [1]
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- Product number
- ALX-804-121F-T100 - Provider product page
- Provider
- Enzo Life Sciences
- Product name
- Granzyme B (human) monoclonal antibody (B18.1) (FITC conjugate)
- Antibody type
- Monoclonal
- Antigen
- Recombinant protein fragment
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Green dye
- Isotype
- IgG
- Antibody clone number
- B18.1
- Vial size
- 100 tests
- Storage
- +4°C
- Handling
- Avoid freeze/thaw cycles. Protect from light.
Submitted references Granzyme-mediated cytotoxicity does not involve the mannose 6-phosphate receptors on target cells.
Acquisition of granzyme B and Fas ligand proteins by human keratinocytes contributes to epidermal cell defense.
Expansion of a peripheral blood perforin+ CD8+ T-cell subset in long-term surviving lung transplanted patients.
Granzyme B and perforin lytic proteins are expressed in CD34+ peripheral blood progenitor cells mobilized by chemotherapy and granulocyte colony-stimulating factor.
Granzyme B and perforin can be used as predictive markers of acute rejection in heart transplantation.
Perforin and granzyme B expression is associated with severe acute rejection. Evidence for in situ localization in alveolar lymphocytes of lung-transplanted patients.
Dressel R, Raja SM, Höning S, Seidler T, Froelich CJ, von Figura K, Günther E
The Journal of biological chemistry 2004 May 7;279(19):20200-10
The Journal of biological chemistry 2004 May 7;279(19):20200-10
Acquisition of granzyme B and Fas ligand proteins by human keratinocytes contributes to epidermal cell defense.
Berthou C, Michel L, Soulié A, Jean-Louis F, Flageul B, Dubertret L, Sigaux F, Zhang Y, Sasportes M
Journal of immunology (Baltimore, Md. : 1950) 1997 Dec 1;159(11):5293-300
Journal of immunology (Baltimore, Md. : 1950) 1997 Dec 1;159(11):5293-300
Expansion of a peripheral blood perforin+ CD8+ T-cell subset in long-term surviving lung transplanted patients.
Berthou C, Legros-Maïda S, Soulié A, Wargnier A, Guillet J, Lafaurie C, Stern M, Sasportes M, Israël-Biet D
Transplantation proceedings 1996 Jun;28(3):1964-7
Transplantation proceedings 1996 Jun;28(3):1964-7
Granzyme B and perforin lytic proteins are expressed in CD34+ peripheral blood progenitor cells mobilized by chemotherapy and granulocyte colony-stimulating factor.
Berthou C, Marolleau JP, Lafaurie C, Soulié A, Dal Cortivo L, Bourge JF, Benbunan M, Sasportes M
Blood 1995 Nov 1;86(9):3500-6
Blood 1995 Nov 1;86(9):3500-6
Granzyme B and perforin can be used as predictive markers of acute rejection in heart transplantation.
Legros-Maïda S, Soulié A, Benvenuti C, Wargnier A, Vallée N, Berthou C, Guillet J, Sasportes M, Sigaux N
European journal of immunology 1994 Jan;24(1):229-33
European journal of immunology 1994 Jan;24(1):229-33
Perforin and granzyme B expression is associated with severe acute rejection. Evidence for in situ localization in alveolar lymphocytes of lung-transplanted patients.
Clément MV, Legros-Maïda S, Israël-Biet D, Carnot F, Soulié A, Reynaud P, Guillet J, Gandjbakch I, Sasportes M
Transplantation 1994 Feb;57(3):322-6
Transplantation 1994 Feb;57(3):322-6
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Supportive validation
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- Enzo Life Sciences (provider)
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- Experimental details
- Detection of granzyme B in permeabilized human CD8+ T lymphocytes (filled profile). Isotype control in combination with a secondary FITC-labeled antibody is shown as control. Method:Permeabilized human T cells (5 x 10E5) were incubated on ice for 30 min in 50 µl FACS buffer (PBS, 5% Fetal calf serum, 0.02% azide) containing 1µg of FITC- labelled B18.1 antibody. After washing in FACS buffer, cells were anlyzed by flow cytometry.
