ALX-804-121-C100

antibody from Enzo Life Sciences

Targeting: GZMB CCPI, CGL-1, CGL1, CSP-B, CSPB, CTLA1, CTSGL1, HLP, SECT

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Flow cytometry (Recommended by provider)

Antibody Data

Product number

ALX-804-121-C100

Provider

Enzo Life Sciences

Proper citation

Enzo Life Sciences Cat#ALX-804-121-C100, RRID:AB_2051556

Product name

Granzyme B (human) monoclonal antibody (B18.1)

Antibody type

Monoclonal

Antigen

Recombinant protein fragment

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

B18.1

Vial size

100 μg

Storage

+4°C

Handling

Avoid freeze/thaw cycles.

References

Submitted references

Granzyme-mediated cytotoxicity does not involve the mannose 6-phosphate receptors on target cells.

Dressel R, Raja SM, Höning S, Seidler T, Froelich CJ, von Figura K, Günther E
The Journal of biological chemistry 2004 May 7;279(19):20200-10

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Acquisition of granzyme B and Fas ligand proteins by human keratinocytes contributes to epidermal cell defense.

Berthou C, Michel L, Soulié A, Jean-Louis F, Flageul B, Dubertret L, Sigaux F, Zhang Y, Sasportes M
Journal of immunology (Baltimore, Md. : 1950) 1997 Dec 1;159(11):5293-300

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Expansion of a peripheral blood perforin+ CD8+ T-cell subset in long-term surviving lung transplanted patients.

Berthou C, Legros-Maïda S, Soulié A, Wargnier A, Guillet J, Lafaurie C, Stern M, Sasportes M, Israël-Biet D
Transplantation proceedings 1996 Jun;28(3):1964-7

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Granzyme B and perforin lytic proteins are expressed in CD34+ peripheral blood progenitor cells mobilized by chemotherapy and granulocyte colony-stimulating factor.

Berthou C, Marolleau JP, Lafaurie C, Soulié A, Dal Cortivo L, Bourge JF, Benbunan M, Sasportes M
Blood 1995 Nov 1;86(9):3500-6

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Granzyme B and perforin can be used as predictive markers of acute rejection in heart transplantation.

Legros-Maïda S, Soulié A, Benvenuti C, Wargnier A, Vallée N, Berthou C, Guillet J, Sasportes M, Sigaux N
European journal of immunology 1994 Jan;24(1):229-33

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Perforin and granzyme B expression is associated with severe acute rejection. Evidence for in situ localization in alveolar lymphocytes of lung-transplanted patients.

Clément MV, Legros-Maïda S, Israël-Biet D, Carnot F, Soulié A, Reynaud P, Guillet J, Gandjbakch I, Sasportes M
Transplantation 1994 Feb;57(3):322-6

Immunocytochemistry

Supportive validation

Submitted by

Enzo Life Sciences (provider)

Main image

Experimental details

Detection of recombinant granzyme B. Granzyme B migrates as a 27 kDa species. Method: Recombinant granzyme B was resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and probed with the monoclonal anti-granzyme B antibody B18.1 at 1µg/ml. Proteins were visualized using a peroxidase-conjugated antibody to mouse IgG (SBA) and a chemiluminescence detection system.

Supportive validation

Submitted by

Enzo Life Sciences (provider)

Main image

Experimental details

Detection of granzyme B in a human CD8+ T cell clone. Method: Human T cells were plated onto polylysine treated glass slides, fixed and permeabilized in methanol at -20oC for 5 min, then in acetone at -20oC for 30 sec. After 3 washes in PBS, 0.1% BSA, slides were incubated for 1 hr at room temperature (RT) with 20 µg/ml of B18.4 antibody in PBS, 0.1% BSA. After rinsing in PBS, a FITC-conjugated anti-mouse IgG was added for 30 min., slides washed again in PBS and visualized using a fluorescence microscope.